Reduction of the hydraulic retention time at constant high organic loading rate to reach the microbial limits of anaerobic digestion in various reactor systems

Ziganshin, Ayrat M. and Schmidt, Thomas and Lv, Zuopeng and Richnow, Hans Hermann and Kleinsteuber, Sabine and Nikolausz, Marcell (2016) Reduction of the hydraulic retention time at constant high organic loading rate to reach the microbial limits of anaerobic digestion in various reactor systems. Bioresource Technology, 217. pp. 62-71. ISSN 0960-8524

Abstract

The effects of hydraulic retention time (HRT) reduction at constant high organic loading rate on the activity of hydrogen-producing bacteria and methanogens were investigated in reactors digesting thin stillage. Stable isotope fingerprinting was additionally applied to assess methanogenic pathways. Based on hydA gene transcripts, Clostridiales was the most active hydrogen-producing order in continuous stirred tank reactor (CSTR), fixed-bed reactor (FBR) and anaerobic sequencing batch reactor (ASBR), but shorter HRT stimulated the activity of Spirochaetales. Further decreasing HRT diminished Spirochaetales activity in systems with biomass retention. Based on mcrA gene transcripts, Methanoculleus and Methanosarcina were the predominantly active in CSTR and ASBR, whereas Methanosaeta and Methanospirillum activity
was more significant in stably performing FBR. Isotope values indicated the predominance of aceticlastic pathway in FBR. Interestingly, an increased activity of Methanosaeta was observed during shortening HRT in CSTR and ASBR despite high organic acids concentrations, what was supported by stable isotope data.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty / Department / School: No Faculty
Date Deposited: 24 May 2017 06:14
Last Modified: 25 May 2017 03:15
Uncontrolled Keywords: biogas; thin stillage; Fe–Fe-hydrogenases; methanogenic activity Stable isotope fingerprinting
Fields of Research : 09 Engineering > 0907 Environmental Engineering > 090703 Environmental Technologies
Identification Number or DOI: 10.1016/j.biortech.2016.01.096
URI: http://eprints.usq.edu.au/id/eprint/32446

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