Monitoring functions in managed microbial systems by cytometric bar coding

Koch, Christin and Fetzer, Ingo and Schmidt, Thomas and Harms, Hauke and Muller, Susann (2013) Monitoring functions in managed microbial systems by cytometric bar coding. Environmental Science and Technology, 47 (3). pp. 1753-1760. ISSN 1520-5851

Abstract

Cytometric monitoring of microbial community dynamics can be used to estimate stability of technical microbial processes like biogas production by analysis of segregated cell abundance changes. In this study, structure variations of a biogas community were cytometrically recorded over 9 months and found to be of diagnostic value for process details. The reactor regime was intentionally disturbed with regard to substrate overload or H2S accumulation. A single-cell based approach called cytometric bar coding (CyBar) for fast identification of reactive subcommunities was used. Functionality of specific subcommunities was uncovered by processing CyBar data with abiotic reactor parameters using Spearman's correlation coefficient. Twenty subcommunities showed a discrete and divergent behavior. For example, a 4-fold substrate overload increased the cell number of two acidogenic index subcommunities to 176 and 193% within three days. Supplementary analyses were done using DNA fingerprinting, cloning, and sequencing. Bioreactor perturbations were shown to create cell abundance changes in subcommunities rather than variations in their phylogenetic composition. The used workflow and macros are ready-to-use tools and allow on-site monitoring and interpretation of variation in microbial community functions within a few hours.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty / Department / School: No Faculty
Date Deposited: 06 Jun 2017 04:42
Last Modified: 27 Jun 2017 04:21
Uncontrolled Keywords: Acidogenic; Bar coding; Biogas production; Cell abundance; Cell number; Correlation coefficient; Diagnostic value; DNA fingerprinting; Microbial communities; Microbial process; Microbial system; Monitoring functions; On-site monitoring; Reactor parameters; Structure variation; Bioengineering; Gas Fuels; Data Processing; Microbiology: Bacteriology, Mycology, Parasitology and Virology; Gram-positive rods and cocci
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060599 Microbiology not elsewhere classified
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: 10.1021/es3041048
URI: http://eprints.usq.edu.au/id/eprint/32372

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