Rare Pyrenophora teres hybridization events revealed by development of sequence-specific PCR markers

Poudel, Barsha and Ellwood, Simon R. and Testa, Alison C. and McLean, Mark and Sutherland, Mark and Martin, Anke (2017) Rare Pyrenophora teres hybridization events revealed by development of sequence-specific PCR markers. Phytopathology, 107 (7). pp. 878-884. ISSN 0031-949X

Abstract

Pyrenophora teres f. teres and P. teres f. maculata cause net form and spot form, respectively, of net blotch on barley (Hordeum vulgare). The two forms reproduce sexually, producing hybrids with genetic and pathogenic variability. Phenotypic identification of hybrids is challenging because lesions induced by hybrids on host plants resemble lesions induced by either P. teres f. teres or P. teres f. maculata. In this study, 12 sequence-specific polymerase chain reaction markers were developed based on expressed regions spread across the genome. The primers were validated using 210 P. teres isolates, 2 putative field hybrids (WAC10721 and SNB172), 50 laboratory-produced hybrids, and 7 isolates collected from barley grass (H. leporinum). The sequence-specific markers confirmed isolate WAC10721 as a hybrid. Only four P. teres f. teres markers amplified on DNA of barley grass isolates. Amplified fragment length polymorphism markers suggested that P. teres barley grass isolates are genetically different from P. teres barley isolates and that the second putative hybrid (SNB172) is a barley grass isolate. We developed a suite of markers which clearly distinguish the two forms of P. teres and enable unambiguous identification of hybrids.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - No Department
Date Deposited: 17 Oct 2017 03:54
Last Modified: 09 Jan 2018 05:40
Uncontrolled Keywords: Molecular Markers, Pyrenophora teres, Hybridisation
Fields of Research : 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Identification Number or DOI: 10.1094/PHYTO-11-16-0396-R
URI: http://eprints.usq.edu.au/id/eprint/32368

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