Characterization of aggregates produced by the potential mycoherbistat Plectosporium alismatisin submerged culture: germination, UV-radiation tolerance and infectivity

Cliquet, Sophie and Despreaux, Julien and Zeeshan, Kashif and de la Broise, Denis and Ash, Gavin (2011) Characterization of aggregates produced by the potential mycoherbistat Plectosporium alismatisin submerged culture: germination, UV-radiation tolerance and infectivity. Biocontrol Science and Technology, 21 (10). pp. 1243-1256. ISSN 0958-3157

Abstract

Plectosporium alismatis, a potential mycoherbistat of Alismatacae spp., has been previously shown to produce aggregates which contain chlamydospores in liquid culture. Weevaluated the impact of medium composition on the formation and composition of aggregates. In shake flasks cultures using 5.74 gL−1 sodium nitrate, 8.8 gL−1 malt extract or glucose and 0.1% Tween 80, P alismatis formed small, uniform (diameter of 75% aggregates <720 μm), dense, melanised aggregates containing 104 conidia and 103 chlamydospores, these numbers remained unchanged during growth. All 7-day-old aggregates exposed to desiccation or/ and UV-radiation germinated. In bioassays using leaf discs of Alisma plantago-aquatica, P. alismatis aggregates caused necrosis, regardless of whether aggregates had been exposed to desiccation and/or UV-radiation prior to application on leaf discs, whereas other propagules (103 propagules disc−1) exposed to drying and UV-radiation stress were unable to cause necrosis. This preliminary research shows the potential of aggregates to be used as part of a formulation of biocontrol agents, provided adequate conditions for optimal aggregate yields are found.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty / Department / School: No Faculty
Date Deposited: 27 Jun 2017 01:26
Last Modified: 27 Jun 2017 01:26
Uncontrolled Keywords: aggregates; air-drying; bioherbistat; UV-radiation;
Fields of Research : 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Identification Number or DOI: 10.1080/09583157.2011.604124
URI: http://eprints.usq.edu.au/id/eprint/32076

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