First comprehensive phylogenetic analysis of the genus Erysiphe (Erysiphales, Erysiphaceae) I. The Microsphaera lineage

Takamatsu, Susumu and Ito, Hanako and Shiroya, Yoshiaki and Kiss, Levente and Heluta, Vasyl (2015) First comprehensive phylogenetic analysis of the genus Erysiphe (Erysiphales, Erysiphaceae) I. The Microsphaera lineage. Mycologia, 107 (3). pp. 475-489. ISSN 0027-5514

Abstract

The genus Erysiphe (including powdery mildew fungi only known as anamorph, Pseudoidium) is the largest genus in the Erysiphaceae and contains more than 50% of all species in this family. Little is known about the phylogenetic structure of this genus. We conducted a comprehensive phylogenetic analysis of the Microsphaera-lineage, a monophyletic group including species of sects. Microsphaera and Erysiphe, using 401 sequences of nuc ITS1-5.8S-ITS2 and the 28S rDNA regions. This analysis gave many small clades delimited by the host plant genus or family. We identified two deep branches, albeit with moderate bootstrap supports, that divided the 401 sequences into three large groups. In addition, we identified four large clades consisting of homogeneous sequences of powdery mildews from a wide range of host plants beyond family level, namely, the E. aquilegiae clade, the E. alphitoides clade, the E. quercicola clade, and the E. trifoliorum s. lat. clade. Isolates from herbaceous plants were mostly situated in the E. aquilegiae clade and in Group III that was located at the most derived position of the Microsphaeralineage. On the other hand, the basal part of the Microsphaera-lineage was occupied by isolates from woody plants except for E. glycines that was used as an outgroup taxon. This supports our previous hypothesis that tree-parasitic powdery mildews are phylogenetically primitive in the Erysiphaceae in general, and host-shift from trees to herbs occurred many times independently during the evolution of powdery mildews. Molecular clock analyses suggested that the divergence of the Microsphaera-lineage began ca. 20 million years ago in the Miocene Epoch of the Neogene Period.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restricted access to Published version due to publisher copyright policy. However, article can be freely accessed through the publisher's website at: http://www.tandfonline.com/doi/full/10.3852/15-007
Faculty / Department / School: No Faculty
Date Deposited: 31 May 2017 01:52
Last Modified: 31 May 2017 03:26
Uncontrolled Keywords: 28S rDNA; host relationship; ITS; Powdery mildew; Pseudoidium; Ascomycota; Fungal DNA; Ribosomal DNA; molecular evolution; molecular sequence data; mycological typing techniques; phylogeny; plant diseases; genetics and molecular biology; eukaryotic genetics; molecular evolution and speciation; Myxomycete and fungal genetics; fungi and fungal associations
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060505 Mycology
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: 10.3852/15-007
URI: http://eprints.usq.edu.au/id/eprint/31995

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