Puccinia komarovii var. glanduliferae var. nov.: a fungal agent for the biological control of Himalayan balsam (Impatiens glandulifera)

Tanner, Robert A. and Ellison, Carol A. and Seier, Marion K. and Kovacs, Gabor M. and Kassai-Jager, Edit and Bereczky, Zsolt and Varia, Sonal and Djeddour, Djami and Singh, Mool Chand and Csiszar, Agnes and Csontos, Péter and Kiss, Levente and Evans, Harry C. (2014) Puccinia komarovii var. glanduliferae var. nov.: a fungal agent for the biological control of Himalayan balsam (Impatiens glandulifera). European Journal of Plant Pathology, 141 (2). pp. 247-266. ISSN 0929-1873

Abstract

Clearly defining the taxonomic identity of a potential biocontrol agent is an essential component of any biological control programme. As part of such a programme against Impatiens glandulifera, a highly invasive annual weed in both Europe and North America, the rust fungus Puccinia komarovii was collected on this host plant in its native Himalayan range. However, current literature indicates that P. komarovii is a pathogen of a number of Impatiens species globally and was described originally from I. parviflora, a species native to Asia and now naturalized in Europe. Morphological comparisons, based on urediniospore and teliospore measurements, were generally inconclusive in showing any clear differences between the accession from I. glandulifera and those from other Impatiens species. Both, nrDNA ITS and ITS2-LSU sequence analyses indicated a difference between the rust infecting I. glandulifera compared to accessions on other hosts. However, the large variations in both ITS and 28S (ITS2-LSU) sequences determined within single accessions in this study, makes a clear separation difficult. Cross-inoculation experiments, using one accession of P. komarovii ex I. glandulifera (from India) and two accessions of P. komarovii ex I. parviflora (from China and Hungary), confirmed the specificity of these strains to their original hosts. Two Himalayan Impatiens species, I. scabrida and I. brachycentra, showed varying levels of susceptibility to these rust accessions, where the former was weakly susceptible to all three accessions and the latter was weakly susceptible only to P. komarovii ex I. parviflora (from China). However, commercial cultivars of I. balsamina proved to be fully susceptible to all rust accessions, although this has not been demonstrated under field conditions in India. Based on these host specificity differences between the rust accessions, we propose a new variety: Puccinia komarovii var. glanduliferae var. nov. associated with I. glandulifera in the Himalayas.


Statistics for USQ ePrint 31994
Statistics for this ePrint Item
Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restricted access to Published version due to publisher copyright policy. Erratum citation: Tanner, Robert A. and Ellison, Carol A. and Seier, Marion K. and Varia, Sonal and Djeddour, Djami and Evans, Harry C. and Kovács, Gábor M. and Kassai-Jáger, Edit and Berecky, Zsolt and Kiss, Levente and Singh, Mool Chand and Csiszár, Ágnes and Csontos, Péter (2015) Erratum: Puccinia komarovii var. glanduliferae var. nov.: a fungal agent for the biological control of Himalayan balsam (Impatiens glandulifera) [Eur J Plant Pathol, DOI 10.1007/s10658-014-0539-x]. European Journal of Plant Pathology, 142 (4). 893-. ISSN 09291873.
Faculty / Department / School: No Faculty
Date Deposited: 31 May 2017 05:29
Last Modified: 08 Jun 2017 06:40
Uncontrolled Keywords: cross inoculation studies; invasive weeds; ITS and 28S sequence analyses; nrDNA variation; rust fungi; taxonomy; Impatiens glandulifera; P. komarovii; pathogen; I. parviflora
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060503 Microbial Genetics
06 Biological Sciences > 0605 Microbiology > 060505 Mycology
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: 10.1007/s10658-014-0539-x
URI: http://eprints.usq.edu.au/id/eprint/31994

Actions (login required)

View Item Archive Repository Staff Only