No indication of strict host associations in a widespread mycoparasite: Grapevine Powdery Mildew (Erysiphe necator) is attacked by phylogenetically distant Ampelomyces strains in the field

Pintye, Alexandra and Bereczky, Zsolt and Kovacs, Gabor M. and Nagy, Laszlo G. and Xu, Xiangming and Legler, Sara Elisabetta and Vaczy, Zsuzsanna and Vaczy, Kalman Zoltan and Caffi, Tito and Rossi, Vittorio and Kiss, Levente (2012) No indication of strict host associations in a widespread mycoparasite: Grapevine Powdery Mildew (Erysiphe necator) is attacked by phylogenetically distant Ampelomyces strains in the field. Phytopathology, 102 (7). pp. 707-716. ISSN 0031-949X

Abstract

Pycnidial fungi belonging to the genus Ampelomyces are common intracellular mycoparasites of powdery mildews worldwide. Some strains have already been developed as commercial biocontrol agents (BCAs) of Erysiphe necator and other powdery mildew species infecting important crops. One of the basic, and still debated, questions concerning the tritrophic relationships between host plants, powdery mildew fungi, and Ampelomyces mycoparasites is whether Ampelomyces strains isolated from certain species of the Erysiphales are narrowly specialized to their original mycohosts or are generalist mycoparasites of many powdery mildew fungi. This is also important for the use of Ampelomyces strains as BCAs. To understand this relationship, the nuclear ribosomal DNA internal transcribed spacer (ITS) and partial actin gene (act1) sequences of 55 Ampelomyces strains from E. necator were analyzed together with those of 47 strains isolated from other powdery mildew species. These phylogenetic analyses distinguished five major clades and strains from E. necator that were present in all but one clade. This work was supplemented with the selection of nine inter-simple sequence repeat (ISSR) markers for strain-specific identification of Ampelomyces mycoparasites to monitor the environmental fate of strains applied as BCAs. The genetic distances among strains calculated based on ISSR patterns have also highlighted the genetic diversity of Ampelomyces mycoparasites naturally occurring in grapevine powdery mildew. Overall, this work showed that Ampelomyces strains isolated from E. necator are genetically diverse and there is no indication of strict mycohost associations in these strains. However, these results cannot rule out a certain degree of quantitative association between at least some of the Ampelomyces lineages identified in this work and their original mycohosts.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restricted access to Published version due to publisher copyright policy. However, this article is freely available from the publisher's website at: http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO-10-11-0270
Faculty / Department / School: No Faculty
Date Deposited: 26 May 2017 01:53
Last Modified: 26 May 2017 01:53
Uncontrolled Keywords: spacer DNA; comparative study; Deuteromycetes; fungal gene; genetic marker; genetic variability; genetics; host range; microbiology; physiology; plant disease; intergenic DNA; genes; fungal; genetic markers; genetic variation; host specificity; mitosporic fungi; plant diseases; Ascomycetes; Erysiphales; Erysiphe necator; fungi; Vitis
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060505 Mycology
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: 10.1094/PHYTO-10-11-0270
URI: http://eprints.usq.edu.au/id/eprint/31887

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