Characterizing the Saltol quantitative trait locus for salinity tolerance in rice

Thomson, Michael J. and de Ocampo, Marjorie and Egdane, James and Rahman, M. Akhlasur and Sajise, Andres Godwin and Adorada, Dante L. and Tumimbang-Raiz, Ellen and Blumwald, Eduardo and Seraj, Zeba I. and Singh, Rakesh K. and Gregorio, Glenn B. and Ismail, Abdelbagi M. (2010) Characterizing the Saltol quantitative trait locus for salinity tolerance in rice. Rice, 3 (2-3). pp. 148-160. ISSN 1939-8433

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Abstract

This study characterized Pokkali-derived quantitative trait loci (QTLs) for seedling stage salinity tolerance in preparation for use in marker-assisted breeding. An analysis of 100 SSR markers on 140 IR29/Pokkali recombinant inbred lines (RILs) confirmed the location of the Saltol QTL on chromosome 1 and identified additional QTLs associated with tolerance. Analysis of a series of backcross lines and near-isogenic lines (NILs) developed to better characterize the effect of the Saltol locus revealed that Saltol mainly acted to control shoot Na +/K + homeostasis. Multiple QTLs were required to acquire a high level of tolerance. Unexpectedly, multiple Pokkali alleles at Saltol were detected within the RIL population and between backcross lines, and representative lines were compared with seven Pokkali accessions to better characterize this allelic variation. Thus, while the Saltol locus presents a complex scenario, it provides an opportunity for markerassisted backcrossing to improve salt tolerance of popular varieties followed by targeting multiple loci through QTL pyramiding for areas with higher salt stress.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version made available under Open Access.
Faculty / Department / School: No Faculty
Date Deposited: 02 Nov 2017 04:28
Last Modified: 02 Nov 2017 04:28
Uncontrolled Keywords: allelic variation; MABC; near isogenic lines; rice; salt stress; saltol QTL;
Fields of Research : 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
Identification Number or DOI: 10.1007/s12284-010-9053-8
URI: http://eprints.usq.edu.au/id/eprint/31743

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