Effects of resveratrol supplementation on methotrexate chemotherapy‐induced bone loss

Lee, Alice M. C. and Shandala, Tetyana and Soo, Pei Pei and Su, Yu-Wen and King, Tristan J. and Chen, Ke-Ming and Howe, Peter R. and Xian, Cory J. (2017) Effects of resveratrol supplementation on methotrexate chemotherapy‐induced bone loss. Nutrients, 9 (3).

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Intensive cancer chemotherapy is known to cause bone defects, which currently lack treatments. This study investigated the effects of polyphenol resveratrol (RES) in preventing bone defects in rats caused by methotrexate (MTX), a commonly used antimetabolite in childhood oncology. Young rats received five daily MTX injections at 0.75 mg/kg/day. RES was orally gavaged daily for seven days prior to, and during, five‐day MTX administration. MTX reduced growth plate thickness, primary spongiosa height, trabecular bone volume, increased marrow adipocyte density, and increased mRNA expression of the osteogenic, adipogenic, and osteoclastogenic factors in the tibial bone. RES at 10 mg/kg was found not to affect bone health in normal rats, but to aggravate the bone damage in MTX‐treated rats. However, RES supplementation at 1 mg/kg preserved the growth plate, primary spongiosa, bone volume, and lowered the adipocyte density. It maintained expression of genes involved in osteogenesis and decreased expression of adipogenic and osteoclastogenic factors. RES suppressed osteoclast formation ex vivo of bone marrow cells from the treated rats. These data suggest that MTX can enhance osteoclast and adipocyte formation and cause bone loss, and that RES supplementation at 1 mg/kg may potentially prevent these bone defects.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
Faculty/School / Institute/Centre: No Faculty
Faculty/School / Institute/Centre: No Faculty
Date Deposited: 14 Aug 2017 04:34
Last Modified: 30 Aug 2018 04:56
Uncontrolled Keywords: adipocytes; bone growth arrest; bone loss; bone marrow adiposity; cancer chemotherapy; growth plate; methotrexate; osteoblasts; osteoclasts; resveratrol
Fields of Research (2008): 11 Medical and Health Sciences > 1111 Nutrition and Dietetics > 111199 Nutrition and Dietetics not elsewhere classified
11 Medical and Health Sciences > 1112 Oncology and Carcinogenesis > 111205 Chemotherapy
Fields of Research (2020): 32 BIOMEDICAL AND CLINICAL SCIENCES > 3210 Nutrition and dietetics > 321099 Nutrition and dietetics not elsewhere classified
32 BIOMEDICAL AND CLINICAL SCIENCES > 3211 Oncology and carcinogenesis > 321105 Chemotherapy
Identification Number or DOI: https://doi.org/10.3390/nu9030255
URI: http://eprints.usq.edu.au/id/eprint/31561

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