Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black spot

Glienke, C. and Pereira, O. L. and Stringari, D. and Fabris, J. and Kava-Cordeiro, V. and Galli-Terasawa, L. and Cunnington, J. and Shivas, R. G. ORCID: https://orcid.org/0000-0001-7494-8716 and Groenewald, J. Z. and Crous, P. W. (2011) Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black spot. Persoonia: Molecular Phylogeny and Evolution of Fungi, 26. pp. 47-56. ISSN 0031-5850


We investigated the identity and genetic diversity of more than 100 isolates belonging to Phyllosticta (teleomorph Guignardia), with particular emphasis on Phyllosticta citricarpa and Guignardia mangiferae s.l. occurring on Citrus. Phyllosticta citricarpa is the causal agent of Citrus Black Spot and is subject to phytosanitary legislation in the EU. This species is frequently confused with a taxon generally referred to as G. mangiferae, the presumed teleomorph of P. capitalensis, which is a non-pathogenic endophyte, commonly isolated from citrus leaves and fruits and a wide range of other hosts. DNA sequence analysis of the nrDNA internal transcribed spacer region (ITS1, 5.8S nrDNA, ITS2) and partial translation elongation factor 1-alpha (TEF1), actin and glyceraldehyde-3-phosphate dehydrogenase (GPDH) genes resolved nine clades correlating to seven known, and two apparently undescribed species. Phyllosticta citribraziliensis is newly described as an endophytic species occurring on Citrus in Brazil. An epitype is designated for P. citricarpa from material newly collected in Australia, which is distinct from P. citriasiana, presently only known on C. maxima from Asia. Phyllosticta bifrenariae is newly described for a species causing leaf and bulb spots on Bifrenaria harrisoniae (Orchidaceae) in Brazil. It is morphologically distinct from P. capitalensis, which was originally described from Stanhopea (Orchidaceae) in Brazil; an epitype is designated here. Guignardia mangiferae, which was originally described from Mangifera indica (Anacardiaceae) in India, is distinguished from the non-pathogenic endophyte, P. brazilianiae sp. nov., which is common on M. indica in Brazil. Furthermore, a combined phylogenetic tree revealed the P. capitalensis s.l. clade to be genetically distinct from the reference isolate of G. mangiferae. Several names are available for this clade, the oldest being P. capitalensis. These results suggest that endophytic, non-pathogenic isolates occurring on a wide host range would be more correctly referred to as P. capitalensis. However, more genes need to be analysed to fully resolve the morphological variation still observed within this clade.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: No Faculty
Faculty/School / Institute/Centre: No Faculty
Date Deposited: 22 May 2017 23:01
Last Modified: 22 May 2017 23:01
Uncontrolled Keywords: Guignardia endophyllicola; Guignardia mangiferae; Phyllosticta bifrenariae; Phyllosticta brazilianiae; Phyllosticta capitalensis; Phyllosticta citriasiana; Phyllosticta citribraziliensis; Phyllosticta citricarpa; Taxonomy; APPLIED MICROBIOLOGY AND BIOTECHNOLOGY; MICROBIAL SYMBIOSES AND OTHER INTERACTIONS; Plants; Endophytes; APPLIED MICROBIOLOGY AND BIOTECHNOLOGY; MICROBIAL DISEASES AND PARASITES; Plant Pathology; Fungal pathogens and diseases; ECOLOGICAL AND ENVIRONMENTAL SCIENCES; ECOLOGY OF COMMUNITIES/ECOSYSTEMS; Symbioses and Mutualisms; PLANT SCIENCE; PLANT PATHOLOGY AND SYMBIOSES; Plant Pathology; Fungi - general; PLANT SCIENCE; ENVIRONMENTAL BIOLOGY; Ecology; Non-symbiotic interactions;
Fields of Research (2008): 06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310805 Plant pathology
Socio-Economic Objectives (2008): E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: https://doi.org/10.3767/003158511X569169
URI: http://eprints.usq.edu.au/id/eprint/31515

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