Mahdi, Layla K. and Higgins, Melanie A. and Day, Christopher J. and Tiralongo, Joe and Hartley-Tassell, Lauren E. and Jennings, Michael P. and Gordon, David L. and Paton, Adrienne W. and Paton, James C. and Ogunniyi, Abiodun D. (2017) The pneumococcal alpha-glycerophosphate oxidase enhances nasopharyngeal colonization through binding to host glycoconjugates. EBioMedicine, 18. pp. 236-243.
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Abstract
Streptococcus pneumoniae (the pneumococcus) is a major human pathogen, causing a broad spectrum of diseases including otitis media, pneumonia, bacteraemia and meningitis. Here we examined the role of a potential pneumococcal meningitis vaccine antigen, alpha-glycerophosphate oxidase (SpGlpO), in nasopharyngeal colonization. We found that serotype 4 and serotype 6A strains deficient in SpGlpO have significantly reduced capacity to colonize the nasopharynx of mice, and were significantly defective in adherence to human nasopharyngeal carcinoma cells in vitro. We also demonstrate that intranasal immunization with recombinant SpGlpO significantly protects mice against subsequent nasal colonization by wild type serotype 4 and serotype 6A strains. Furthermore, we show that SpGlpO binds strongly to lacto/neolacto/ganglio host glycan structures containing the GlcNAcβ1-3Galβ disaccharide, suggesting that SpGlpO enhances colonization of the nasopharynx through its binding to host glycoconjugates. We propose that SpGlpO is a promising vaccine candidate against pneumococcal carriage, and warrants inclusion in a multi-component protein vaccine formulation that can provide robust, serotype-independent protection against all forms of pneumococcal disease.
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| Item Type: | Article (Commonwealth Reporting Category C) |
|---|---|
| Refereed: | Yes |
| Item Status: | Live Archive |
| Faculty/School / Institute/Centre: | No Faculty |
| Faculty/School / Institute/Centre: | No Faculty |
| Date Deposited: | 17 Oct 2017 22:46 |
| Last Modified: | 17 Apr 2018 23:05 |
| Uncontrolled Keywords: | bacterial pathogens, Streptococcus pneumoniae; protein vaccines; pneumococcal disease; colonization; adherence; host glycoconjugates; alpha-glycerophosphate oxidase; immunization |
| Fields of Research (2008): | 06 Biological Sciences > 0605 Microbiology > 060501 Bacteriology |
| Fields of Research (2020): | 31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310701 Bacteriology |
| Socio-Economic Objectives (2008): | E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences |
| Identification Number or DOI: | https://doi.org/10.1016/j.ebiom.2017.03.002 |
| URI: | http://eprints.usq.edu.au/id/eprint/30946 |
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