Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

Vagi, Pal and Caffi, Tito and Vaczy, Kalman Zoltan and Nemeth, Mark Z. and Kiss, Levente (2016) Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator. European Journal of Plant Pathology, 144 (4). pp. 799-802. ISSN 0929-1873

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The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Accepted version deposited in accordance with the copyright policy of the publisher.
Faculty/School / Institute/Centre: Current - Institute for Life Sciences and the Environment - Centre for Crop Health
Date Deposited: 27 Feb 2017 05:05
Last Modified: 01 Nov 2017 04:41
Uncontrolled Keywords: grapevine powdery mildew, fluorescein diacetate, ethidium bromide, propidium iodide, epifluorescence microscopy
Fields of Research : 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Funding Details:
Identification Number or DOI: 10.1007/s10658-015-0797-2

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