Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

Vagi, Pal and Caffi, Tito and Vaczy, Kalman Zoltan and Nemeth, Mark Z. and Kiss, Levente (2016) Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator. European Journal of Plant Pathology, 144 (4). pp. 799-802. ISSN 0929-1873

[img]
Preview
Text (Accepted version)
EJPP-D-15-00368_R1.pdf

Download (863Kb) | Preview

Abstract

The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.


Statistics for USQ ePrint 30819
Statistics for this ePrint Item
Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Accepted version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Current - Institute for Agriculture and the Environment
Date Deposited: 27 Feb 2017 05:05
Last Modified: 01 Nov 2017 04:41
Uncontrolled Keywords: grapevine powdery mildew, fluorescein diacetate, ethidium bromide, propidium iodide, epifluorescence microscopy
Fields of Research : 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Funding Details:
Identification Number or DOI: 10.1007/s10658-015-0797-2
URI: http://eprints.usq.edu.au/id/eprint/30819

Actions (login required)

View Item Archive Repository Staff Only