Routes of Hendra virus excretion in naturally infected flying-foxes: implications for viral transmission and spillover risk

Edson, Daniel and Field, Hume and McMichael, Lee and Vidgen, Miranda and Goldspink, Lauren and Broos, Alice and Melville, Deb and Kristoffersen, Joanna and de Jong, Carol and McLaughlin, Amanda and Davis, Rodney and Kung, Nina and Jordan, David and Kirkland, Peter and Smith, Craig (2015) Routes of Hendra virus excretion in naturally infected flying-foxes: implications for viral transmission and spillover risk. PLoS One, 10 (10). pp. 1-15.

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Pteropid bats or flying-foxes (Chiroptera: Pteropodidae) are the natural host of Hendra virus (HeV) which sporadically causes fatal disease in horses and humans in eastern Australia. While there is strong evidence that urine is an important infectious medium that likely drives bat to bat transmission and bat to horse transmission, there is uncertainty about the relative importance of alternative routes of excretion such as nasal and oral secretions, and faeces. Identifying the potential routes of HeV excretion in flying-foxes is important to effectively mitigate equine exposure risk at the bat-horse interface, and in determining transmission rates in host-pathogen models. The aim of this study was to identify the major routes of HeV excretion in naturally infected flying-foxes, and secondarily, to identify between-species variation in excretion prevalence. A total of 2840 flying-foxes from three of the four Australian mainland species (Pteropus alecto, P. poliocephalus and P. scapulatus) were captured and sampled at multiple roost locations in the eastern states of Queensland and New South Wales between 2012 and 2014. A range of biological samples (urine and serum, and urogenital, nasal, oral and rectal swabs) were collected from anaesthetized bats, and tested for HeV RNA using a qRT-PCR assay targeting the M gene. Forty-two P. alecto (n = 1410) had HeV RNA detected in at least one sample, and yielded a total of 78 positive samples, at an overall detection rate of 1.76% across all samples tested in this species (78/4436). The rate of detection, and the amount of viral RNA, was highest in urine samples (>serum, packed haemocytes >faecal >nasal >oral), identifying urine as the most plausible source of infection for flying-foxes and for horses. Detection in a urine sample was more efficient than detection in urogenital swabs, identifying the former as the preferred diagnostic sample. The detection of HeV RNA in serum is consistent with haematogenous spread, and with hypothesised latency and recrudesence in flying-foxes. There were no detections in P. poliocephalus (n = 1168 animals; n = 2958 samples) or P. scapulatus (n = 262 animals; n = 985 samples), suggesting (consistent with other recent studies) that these species are epidemiologically less important than P. alecto in HeV infection dynamics. The study is unprecedented in terms of
the individual animal approach, the large sample size, and the use of a molecular assay to directly determine infection status. These features provide a high level of confidence in the veracity of our findings, and a sound basis from which to more precisely target equine risk mitigation strategies.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version made available in accordance with Creative Commons Attribution License 4.0.
Faculty / Department / School: Current - Institute for Agriculture and the Environment
Date Deposited: 28 Feb 2017 00:17
Last Modified: 06 Apr 2017 05:46
Uncontrolled Keywords: urine; horses; excretion; equine; bats; serology; secretion; viral transmission; infection
Fields of Research : 07 Agricultural and Veterinary Sciences > 0707 Veterinary Sciences > 070705 Veterinary Immunology
07 Agricultural and Veterinary Sciences > 0707 Veterinary Sciences > 070704 Veterinary Epidemiology
07 Agricultural and Veterinary Sciences > 0707 Veterinary Sciences > 070712 Veterinary Virology
Identification Number or DOI: 10.1371/journal.pone.0140670

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