Activation of the selenoprotein SEPS1 gene expression by pro-inflammatory cytokines in HepG2 cells

Gao, Yuan and Hannan, Nicholas R. F. and Wanyonyi, Stephen and Konstantopolous, Nicky and Pagnon, Joanne and Feng, Helen C. and Jowett, Jeremy B. M. and Kim, Kee-Hong and Walder, Ken and Collier, Greg R. (2006) Activation of the selenoprotein SEPS1 gene expression by pro-inflammatory cytokines in HepG2 cells. Cytokine, 33 (5). pp. 246-251. ISSN 1043-4666

Abstract

SEPS1 (also called selenoprotein S, SelS) plays an important role in the production of inflammatory cytokines and its expression is activated by endoplasmic reticulum (ER) stress. In this report, we have identified two binding sites for the nuclear factor kappa B in the human SEPS1 promoter. SEPS1 gene expression, protein levels and promoter activity were all increased 2-3-fold by TNF-alpha and IL-1beta in HepG2 cells. We have also confirmed that the previously proposed ER stress response element GGATTTCTCCCCCGCCACG in the SEPS1 proximate promoter is fully functional and responsive to ER stress. However, concurrent treatment of HepG2 cells with IL-1beta and ER stress produced no additive effect on SEPS1 gene expression. We conclude that SEPS1 is a new target gene of NF-kappaB. Together with our previous findings that SEPS1 may regulate cytokine production in macrophage cells, we propose a regulatory loop between cytokines and SEPS1 that plays a key role in control of the inflammatory response.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty / Department / School: Current - Institute for Agriculture and the Environment
Date Deposited: 18 Oct 2017 02:05
Last Modified: 18 Oct 2017 02:05
Uncontrolled Keywords: selenoprotein; glucose-regulated protein; ER stress; cytokine; gene expression
Fields of Research : 11 Medical and Health Sciences > 1115 Pharmacology and Pharmaceutical Sciences > 111502 Clinical Pharmacology and Therapeutics
11 Medical and Health Sciences > 1115 Pharmacology and Pharmaceutical Sciences > 111501 Basic Pharmacology
Socio-Economic Objective: C Society > 92 Health > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy
Identification Number or DOI: 10.1016/j.cyto.2006.02.005
URI: http://eprints.usq.edu.au/id/eprint/30726

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