Tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity

Wanyonyi, Stephen S. and Sharp, Julie A. and Khalil, Elie and Lefevre, Christophe and Nicholas, Kevin R. (2011) Tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 160 (3). pp. 431-439. ISSN 1095-6433

Abstract

Cathelicidins secreted in milk may be central to autocrine feedback in the mammary gland for optimal development in addition to conferring innate immunity to both the mammary gland and the neonate. This study exploits the unique reproductive strategy of the tammar wallaby (Macro pus eugenii) model to analyse differential splicing of cathelicidin genes and to evaluate the bactericidal activity and effect of the protein on mammary epithelial cell proliferation. Two linear peptides, Con73 and Con218, derived from the heterogeneous carboxyl end of cathelicidin transcripts, MaeuCath1 and MaeuCath7 respectively, were evaluated for antimicrobial activity. Both Con73 and Con218 significantly inhibited the growth of Staphylococcus aureus, Pseudomonas aureginosa, Enterococcus faecalis and Salmonella enterica. In addition both MaeuCath1 and MaeuCath7 stimulated proliferation of primary tammar wallaby mammary epithelial cells (WallMEC). Lactation-phase specific alternate spliced transcripts were determined for MaeuCath1 showing utilisation of both antimicrobial and proliferative functions are required by the mammary gland and the suckled young. The study has shown for the first time that temporal regulation of milk cathelicidins may be crucial in antimicrobial protection of the mammary gland and suckled young and mammary cell proliferation.


Statistics for USQ ePrint 30601
Statistics for this ePrint Item
Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty / Department / School: No Faculty
Date Deposited: 24 Feb 2017 06:39
Last Modified: 15 Mar 2017 04:08
Uncontrolled Keywords: cathelicidin; tammar wallaby; mammary epithelial cells; cell proliferation; antimicrobial;
Fields of Research : 11 Medical and Health Sciences > 1116 Medical Physiology > 111601 Cell Physiology
11 Medical and Health Sciences > 1107 Immunology > 110707 Innate Immunity
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences
Identification Number or DOI: 10.1016/j.cbpa.2011.07.015
URI: http://eprints.usq.edu.au/id/eprint/30601

Actions (login required)

View Item Archive Repository Staff Only