The extracellular matrix regulates MaeuCath1a gene expression

Wanyonyi, Stephen S. and Lefevre, Christophe and Sharp, Julie A. and Nicholas, Kevin R. (2013) The extracellular matrix regulates MaeuCath1a gene expression. Developmental and Comparative Immunology, 40 (3-4). pp. 289-299. ISSN 0145-305X


We have previously shown that the gene for MaeuCath1, a cathelicidin secreted in wallaby milk is alternately spliced into two variants, MaeuCath1a and MaeuCath1b which are temporally regulated in order to provide antimicrobial protection to the newborn and stimulate mammary growth, respectively. The current study investigated the extracellular matrix (ECM) for its regulatory role in MaeuCath1 gene expression. Reverse transcription qPCR using RNA isolated from mammary epithelial cells (WallMEC) cultured on ECM showed that ECM regulates MaeuCath1a gene expression in a lactation phase-dependent manner. Luciferase reporter-based assays and in silico analysis of deletion fragments of the 2245bp sequence upstream of the translation start site identified ECM-dependent positive regulatory activity in the -709 to -15 region and repressor activity in the -919 to -710 region. Electrophoretic Gel Mobility Shift Assays (EMSA) using nuclear extract from ECM-treated WallMEC showed differential band shift in the -839 to -710 region.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty/School / Institute/Centre: Historic - Institute for Agriculture and the Environment
Date Deposited: 23 Feb 2017 04:57
Last Modified: 23 Feb 2017 05:40
Uncontrolled Keywords: extracellular matrix; mammary epithelial cells; cathelicidin; lactation; cathelicidin promoter; gene regulation
Fields of Research : 11 Medical and Health Sciences > 1116 Medical Physiology > 111601 Cell Physiology
06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060106 Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)
11 Medical and Health Sciences > 1116 Medical Physiology > 111603 Systems Physiology
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences
Identification Number or DOI: 10.1016/j.dci.2013.02.010

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