Hypoxic exercise training promotes apelin/APJ expression in skeletal muscles of high fat diet-induced obese mice

Ji, Weixiu and Gong, Lijing and Wang, Jianxiong and He, Hui and Zhang, Ying (2017) Hypoxic exercise training promotes apelin/APJ expression in skeletal muscles of high fat diet-induced obese mice. Protein and Peptide Letters, 24 (1). pp. 64-70. ISSN 0929-8665


Apelin, an endogenous ligand of the G-protein-coupled receptor APJ, is a novel myokine and may play a key role in regulating energy metabolism. The purpose of the present study was to investigate the effects of hypoxic exposure, exercise, and hypoxic exercise training on the expression of apelin and APJ in skeletal muscle of obese mice. 60 two-month old C57BL/6J mice were randomly divided into two groups: 10 in normal diet group (N) and 50 in the high fat diet (HFD) groups. After two months of feeding, the HFD mice whose body weight 20% higher than the average weight of N group were selected as obese mice and were further allocated into four groups: Control (C), Exercise (E), Hypoxia (H), and Exercise plus Hypoxia (E+H), at 8-9 mice/group. Besides body weight, measured variables in skeletal muscle were protein/mRNA levels of apelin/APJ, AMPKα-Thr172 phosphorylation, hypoxia inducible factor-1α (HIF-1α), mRNA levels of peroxisome proliferator-activated receptorα (PPARα), estrogen-related receptor (ERRα), and nuclear respiratory factor1 (NRF1). Obese mice had significantly lower mRNA and protein expressions of apelin/APJ in skeletal muscles than the normal body weight mice. After four weeks of interventions, hypoxic exercise training decreased body weight and increased mRNA and protein expressions of apelin and APJ, mRNA expression of ERRα, and protein expression of HIF-1α. These results indicate that changes of body weight may be associated with the levels of apelin/APJ expressions in skeletal muscle.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restricted access to Published version due to publisher copyright policy.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Health and Wellbeing
Date Deposited: 20 Apr 2017 05:57
Last Modified: 11 Apr 2018 02:50
Uncontrolled Keywords: hypoxic exercise training; apelin; APJ; skeletal muscle; obese mice
Fields of Research : 06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: 10.2174/0929866524666161111111726
URI: http://eprints.usq.edu.au/id/eprint/30223

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