Molecular identification of mycorrhizal Russulaceae fungi

Boddington, Morwenna and Dearnaley, John D. and Lebel, Teresa and Leonard, Patrick (2015) Molecular identification of mycorrhizal Russulaceae fungi. In: 2015 Scientific Meeting of the Australasian Mycological Society, 14-16 July 2015, Canberra, Australia.

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Abstract

The Russulaceae is a family of basidiomycetous fungi found in a variety of habitats around the world. While reasonably common in Australian temperate forests, little work has been conducted on this group, with the result that many taxa are undescribed or poorly documented. The aim of
this research project is to improve this situation.
The identification of Russulaceae fruiting bodies to species level has many challenges and is predominantly based on characteristics that are highly subjective, changeable, require specialised chemicals, and specialised equipment. A certain amount of expertise is also required. However, even this is no guarantee of consistently obtaining a positive species identification.
Molecular analysis potentially offers a non-subjective method of identification. In particular, the internal transcribed spacer (ITS) region of fungal DNA has been proposed as the primary standard molecular benchmark for identification of fungi. Coupled with access to millions of sequences held in databases such as GenBank, it would seem that molecular identification offers a useful opportunity to streamline and improve the identification of the Russulaceae. However, this is not always the case, and in sequencing the ITS region of numerous specimens from this family a number of issues have arisen. This presentation will examine some of the difficulties experienced in the molecular identification of Australian Russulaceae species and discuss some of the potential solutions.


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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Lecture)
Refereed: No
Item Status: Live Archive
Additional Information: Oral presentation - only Abstract printed, as provided here.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences
Date Deposited: 07 Dec 2016 23:30
Last Modified: 28 Feb 2017 06:37
Uncontrolled Keywords: mycorrhizal Russulaceae fungi
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060505 Mycology
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
URI: http://eprints.usq.edu.au/id/eprint/30097

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