Molecular identification of fungal endophytes in Dipodium roseum roots

Boddington, M. and Lebel, T. and Leonard, P. and Dearnaley, J. D. W. (2016) Molecular identification of fungal endophytes in Dipodium roseum roots. In: 2016 Scientific Meeting of the Australasian Mycological Society (AMS) with the Fungal Network of New Zealand (FUNNZ), 3-5 May 2016, Queenstown, New Zealand.

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Abstract

Dipodium or hyacinth orchids are large, fleshy, terrestrial orchids commonly seen in summer in Australian woodlands. Most species have limited chlorophyll, appear to be non-photosynthetic and rely on mycorrhizal fungi for their nutrition. Previous molecular investigations of the endophytic fungi of roots of Dipodium variegatum and Dipodium hamiltonianum have shown that the orchids are largely colonised by members of the ectomycorrhizal basidiomycete family, the Russulaceae. The fact that these plants consistently grew close to the base of Eucalyptus and Callitris trees suggested that the orchids acquire carbon and minerals from the tree host via an ectomycorrhizal connection. In the current project the fungal endophytes of Dipodium roseum were investigated for the first time. Orchid roots were sampled from multiple sites over two seasons in southern Queensland, Australia. DNA was extracted from colonised roots and isolated pelotons of the orchid. PCR amplification was conducted using ITS1F and ITS4 primers and following DNA purification, sent for Sanger sequencing at the Australian Genome Research Facility. BLAST searches of returned sequences against the GenBank database revealed a variety of fungi colonising the roots of D. roseum. This included a number of fungi not previously found as root endophytes of Orchidaceae species.


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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Poster)
Refereed: Yes
Item Status: Live Archive
Additional Information: Poster presentation - only Abstract printed, as provided here.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences
Date Deposited: 12 Dec 2016 06:02
Last Modified: 29 May 2017 23:41
Uncontrolled Keywords: Dipodium, Russulaceae, endophytic fungi
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060505 Mycology
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
URI: http://eprints.usq.edu.au/id/eprint/30096

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