Standardizing resistance screening to Pseudomonas fuscovaginae and evaluation of rice germplasm at seedling and adult plant growth stages

Adorada, Dante L. and Stodart, B. J. and Vera Cruz, C. and Gregorio, G. and Pangga, I. and Ash, G. J. (2013) Standardizing resistance screening to Pseudomonas fuscovaginae and evaluation of rice germplasm at seedling and adult plant growth stages. Euphytica, 192 (1). pp. 1-16. ISSN 0014-2336

Abstract

Sheath lesions, grain sterility and grain discolouration of rice caused by Pseudomonas fuscovaginae can cause yield losses of up to 100 %. The most sustainable method of managing this disease is the use of host plant resistance. To identify sources of resistance an inoculation method that is practical, rapid and reliable is needed. We compare three different inoculation methods. Results showed that the pin-prick method is appropriate for identifying sources of resistance to P. fuscovaginae, while the spray method could be useful for mass screening of rice genotypes. The seed-soaking method was also evaluated and has showed potential in detection of early disease resistance. A total of 16 Multiparent Advanced Generation Inter-Crosses and 20 OryzaSNP set varieties from the International Rice Research Institute were evaluated using the pin-prick and seed-soaking methods. All growth stages were susceptible to the pathogen and the 10(7) cfu mL(-1) inoculum concentration was optimal for discriminating between resistant and susceptible genotypes. For the pin-prick method, a single point assessment of disease severity at 14 days post-inoculation could be used instead of the AUDPC values to classify genotypes. An index of reduction in seedling height 10 days after seed soaking was established for the classification of the genotypes reaction to the disease. Resistant varieties identified using both the pin-prick and seed-soaking methods could be verified for use in disease resistance breeding programs. Of the 36 genotypes evaluated 22 were found to be resistant at the late booting or early panicle exsertion stage by pin-prick method, while 25 were resistant at the seed to germination stage. No correlation was found between the resistance classification of varieties between the two inoculation methods, indicating that there could be different mechanisms of resistance to P. fuscovaginae in rice.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences
Date Deposited: 23 Feb 2017 01:37
Last Modified: 28 Mar 2017 06:28
Uncontrolled Keywords: Sheath brown rot;� Inoculation; Disease resistance; � MAGIC; �OryzaSNP; AUDPC
Fields of Research : 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective: B Economic Development > 82 Plant Production and Plant Primary Products > 8204 Summer Grains and Oilseeds > 820402 Rice
Identification Number or DOI: 10.1007/s10681-012-0804-z
URI: http://eprints.usq.edu.au/id/eprint/30041

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