Determining putative vectors of the Bogia Coconut Syndrome Phytoplasma using loop-mediated isothermal amplification of single insect-feeding media

Lu, Hengyu and Wilson, Bree A. L. and Ash, Gavin J. and Woruba, Sharon B. and Fletcher, Murray J. and You, Minsheng and Yang, Guang and Gurr, Geoff M. (2016) Determining putative vectors of the Bogia Coconut Syndrome Phytoplasma using loop-mediated isothermal amplification of single insect-feeding media. Scientific Reports, 6 (35801 ). pp. 1-10.

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Abstract

Phytoplasmas are insect vectored mollicutes responsible for disease in many economically important crops. Determining which insect species are vectors of a given phytoplasma is important for managing disease but is methodologically challenging because disease-free plants need to be exposed to large numbers of insects, often over many months. A relatively new method to detect likely transmission involves molecular testing for phytoplasma DNA in sucrose solution that insects have fed upon. In this study we combined this feeding medium method with a loop-mediated isothermal amplification (LAMP) assay to study 627 insect specimens of 11 Hemiptera taxa sampled from sites in Papua New Guinea affected by Bogia coconut syndrome (BCS). The LAMP assay detected phytoplasma DNA from the feeding solution and head tissue of insects from six taxa belonging to four families: Derbidae, Lophopidae, Flatidae and Ricaniidae. Two other taxa yielded positives only from the heads and the remainder tested negative. These results demonstrate the utility of combining single-insect feeding medium tests with LAMP assays to identify putative vectors that can be the subject of transmission tests and to better understand phytoplasma pathosystems.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version made available under Creative Commons Attribution 4.0 International License.
Faculty / Department / School: Current - Institute for Agriculture and the Environment
Date Deposited: 14 Feb 2017 02:13
Last Modified: 17 Jan 2018 01:43
Uncontrolled Keywords: Bogia Coconut Syndrome (BCS); phytoplasmas; palms; coconut palms; disease management; loop-mediated isothermal amplification (LAMP)
Fields of Research : 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Funding Details:
Identification Number or DOI: 10.1038/srep35801
URI: http://eprints.usq.edu.au/id/eprint/29924

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