Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste

Kiran, Esra Uckun and Trzcinski, Antoine P. and Liu, Yu (2014) Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste. Biofuel Research Journal, 1 (3). pp. 98-105.

Abstract

In this study, food wastes such as waste bread, savory, waste cakes, cafeteria waste, fruits, vegetables and potatoes were used as sole substrate for glucoamylase production by solid state fermentation. Response surface methodology was employed to optimize the fermentation conditions for improving the production of high activity enzyme. It was found that waste cake was the best substrate for glucoamylase production. Among all the parameters studied, glucoamylase activity was significantly affected by the initial pH and incubation time. The highest glucoamylase activity of 108.47 U/gds was achieved at initial pH of 7.9, moisture content of 69.6% wt., inoculum loading of 5.2×105 cells/gram substrate (gs) and incubation time of 6 d. The enzyme preparation could effectively digest 50% suspension of domestic food waste in 24 h with an almost complete saccharification using an enzyme dose of only 2U/g food waste at 60°C.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: c. Green Wave Publishing.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Civil Engineering and Surveying
Date Deposited: 06 Oct 2016 05:16
Last Modified: 06 Oct 2016 05:16
Uncontrolled Keywords: glucoamylase; Aspergillus awamori; food waste; saccharification; solid state fermentation; response surface methodology
Fields of Research : 09 Engineering > 0904 Chemical Engineering > 090499 Chemical Engineering not elsewhere classified
09 Engineering > 0907 Environmental Engineering > 090703 Environmental Technologies
05 Environmental Sciences > 0502 Environmental Science and Management > 050205 Environmental Management
Socio-Economic Objective: D Environment > 96 Environment > 9609 Land and Water Management > 960912 Urban and Industrial Water Management
URI: http://eprints.usq.edu.au/id/eprint/29811

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