Taking transgenic rice drought screening to the field

Gaudin, Amelie C. M. and Henry, Amelia and Sparks, Adam H. ORCID: https://orcid.org/0000-0002-0061-8359 and Slamet-Loedin, Inez H. (2013) Taking transgenic rice drought screening to the field. Journal of Experimental Botany, 64 (1). pp. 109-117. ISSN 0022-0957


Abstract

Numerous transgenes have been reported to increase rice drought resistance, mostly in small-scale experiments under vegetative-stage drought stress, but few studies have included grain yield or field evaluations. Different definitions of drought resistance are currently in use for field-based and laboratory evaluations of transgenics, the former emphasizing plant responses that may not be linked to yield under drought. Although those fundamental studies use efficient protocols to uncover and validate gene functions, screening conditions differ greatly from field drought environments where the onset of drought stress symptoms is slow (2–3 weeks). Simplified screening methods, including severely stressed survival studies, are therefore not likely to identify transgenic events with better yield performance under drought in the target environment. As biosafety regulations are becoming established to allow field trials in some rice-producing countries, there is a need to develop relevant screening procedures that scale from preliminary event selection to greenhouse and field trials. Multilocation testing in a range of drought environments may reveal that different transgenes are necessary for different types of drought-prone field conditions. We describe here a pipeline to improve the selection efficiency and reproducibility of results across drought treatments and test the potential of transgenic rice for the development of drought-resistant material for agricultural purposes.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this record cannot be displayed.
Faculty/School / Institute/Centre: No Faculty
Faculty/School / Institute/Centre: No Faculty
Date Deposited: 04 Oct 2016 00:29
Last Modified: 27 Sep 2019 04:53
Uncontrolled Keywords: drought, field methodology, rice, screening, transgenic
Fields of Research (2008): 06 Biological Sciences > 0607 Plant Biology > 060705 Plant Physiology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
Fields of Research (2020): 31 BIOLOGICAL SCIENCES > 3108 Plant biology > 310806 Plant physiology
30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3004 Crop and pasture production > 300406 Crop and pasture improvement (incl. selection and breeding)
Identification Number or DOI: https://doi.org/10.1093/jxb/ers313
URI: http://eprints.usq.edu.au/id/eprint/29789

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