Identification of virulence associated loci in the emerging broad host range plant pathogen Pseudomonas fuscovaginae

Patel, Hitendra Kumar and Matiuzzo, Maura and Bertani, Iris and Bigirimana, Vincent De Paul and Ash, Gavin J. and Hofte, Monica and Venturi, Vittoria (2014) Identification of virulence associated loci in the emerging broad host range plant pathogen Pseudomonas fuscovaginae. BMC Microbiology, 14 (274). pp. 1-13. ISSN 1471-2180

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Abstract

BACKGROUND: Pseudomonas fuscovaginae (Pfv) is an emerging plant pathogen of rice and also of other gramineae plants. It causes sheath brown rot disease in rice with symptoms that are characterized by brown lesions on the flag leaf sheath, grain discoloration and sterility. It was first isolated as a high altitude pathogen in Japan and has since been reported in several countries throughout the world. Pfv is a broad host range pathogen and very little is known about its virulence mechanisms.

RESULTS: An in planta screen of 1000 random independent Tn5 genomic mutants resulted in the isolation of nine mutants which showed altered virulence. Some of these isolates are mutated for functions which are known to be virulence associated factors in other phytopathogenic bacteria (eg. pil gene, phytotoxins and T6SS) and others might represent novel virulence loci.

CONCLUSIONS: Being an emerging pathogen worldwide, the broad host range pathogen Pfv has not yet been studied for its virulence functions. The roles of the nine loci identified in the in planta screen are discussed in relation to pathogenicity of Pfv. In summary, this article reports a first study on the virulence of this pathogen involving in planta screening studies and suggests the presence of several virulence features with known and novel functions in the Pseudomonas group of bacteria.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2014 Patel et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences
Date Deposited: 22 Sep 2016 01:58
Last Modified: 12 Dec 2016 00:15
Uncontrolled Keywords: DNA Transposable Elements; Gene Knockout Techniques; Japan; Mutagenesis, Insertional; Plant Diseases; Poaceae; Pseudomonas; Virulence
Fields of Research : 06 Biological Sciences > 0607 Plant Biology > 060799 Plant Biology not elsewhere classified
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Identification Number or DOI: 10.1186/s12866-014-0274-7
URI: http://eprints.usq.edu.au/id/eprint/29745

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