Identification of QTLs associated with resistance to phomopsis pod blight (Diaporthe toxica) in Lupinus albus

Cowley, Raymond and Luckett, David J. and Ash, Gavin J. and Harper, John D. I. and Vipin, Cina A. and Raman, Harsh and Ellwood, Simon (2014) Identification of QTLs associated with resistance to phomopsis pod blight (Diaporthe toxica) in Lupinus albus. Breeding Science , 64 (1). pp. 83-89. ISSN 1344-7610

Abstract

Phomopsis blight in Lupinus albus is caused by a fungal pathogen, Diaporthe toxica. It can invade all plant parts, leading to plant material becoming toxic to grazing animals, and potentially resulting in lupinosis. Identifying sources of resistance and breeding for resistance remains the best strategy for controlling Phomopsis and reducing lupinosis risks. However, loci associated with resistance to Phomopsis blight have not yet been identified. In this study, quantitative trait locus (QTL) analysis identified genomic regions associated with resistance to Phomopsis pod blight (PPB) using a linkage map of L. albus constructed previously from an F8 recombinant inbred line population derived from a cross between Kiev-Mutant (susceptible to PPB) and P27174 (resistant to PPB). Phenotyping was undertaken using a detached pod assay. In total, we identified eight QTLs for resistance to PPB on linkage group (LG) 3, LG6, LG10, LG12, LG17 and LG27 from different phenotyping environments. However, at least one QTL, QTL-5 on LG10 was consistently detected in both phenotyping environments and accounted for up to 28.2% of the total phenotypic variance. The results of this study showed that the QTL-2 on LG3 interacts epistatically with QTL-5 and QTL-6, which map on LG10 and LG12, respectively.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty / Department / School: No Faculty
Date Deposited: 15 Sep 2016 06:20
Last Modified: 15 Sep 2016 06:20
Uncontrolled Keywords: DArT, Rqtl, multi-phase experiments, anamorph Phomopsis leptostromiformis, broad-leaf lupin
Fields of Research : 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Identification Number or DOI: 10.1270/jsbbs.64.83
URI: http://eprints.usq.edu.au/id/eprint/29733

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