Biological control of sclerotinia stem rot of canola using antagonistic bacteria

Kamal, M. M. and Lindbeck, K. D. and Savocchia, S. and Ash, G. J. (2015) Biological control of sclerotinia stem rot of canola using antagonistic bacteria. Plant Pathology, 64 (6). pp. 1375-1384. ISSN 0032-0862

Abstract

Stem rot caused by Sclerotinia sclerotiorum is a major fungal disease of canola worldwide. In Australia the management of stem rot relies primarily on strategic application of synthetic fungicides. In an attempt to find alternative strategies for the management of the disease, 514 naturally occurring bacterial isolates were screened for antagonism to S. sclerotiorum. Antifungal activity against mycelial growth of the fungus was exhibited by three isolates of bacteria. The bacteria were identified as Bacillus cereus (SC-1 and P-1) and Bacillus subtilis (W-67) via 16S rRNA sequencing. In vitro antagonism assays using these isolates resulted in significant inhibition of mycelial elongation and complete inhibition of sclerotial germination by both non-volatile and volatile metabolites. The antagonistic strains caused a significant reduction in the viability of sclerotia when tested in a greenhouse pot trial with soil collected from the field. Spray treatments of bacterial strains reduced disease incidence and yielded higher control efficacy both on inoculated cotyledons and stems. Application of SC-1 and W-67 in the field at 10% flowering stage (growth stage 4�00) of canola demonstrated that control efficacy of SC-1 was significantly higher in all three trials (over 2 years) when sprayed twice at 7-day intervals. The greatest control of disease was observed with the fungicide Prosaro� 420SC or with two applications of SC-1. The results demonstrated that, in the light of environmental concerns and increasing cost of fungicides, B. cereus SC-1 may have potential as a biological control agent of sclerotinia stem rot of canola in Australia.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Access to published version in accordance with the copyright policy of the publisher.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences
Date Deposited: 01 Sep 2016 03:33
Last Modified: 04 Jun 2018 06:27
Uncontrolled Keywords: bacteria, biocontrol, canola, Sclerotinia, stem rot
Fields of Research : 06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
06 Biological Sciences > 0605 Microbiology > 060599 Microbiology not elsewhere classified
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Identification Number or DOI: 10.1111/ppa.12369
URI: http://eprints.usq.edu.au/id/eprint/29675

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