Simultaneous selection of wheat lines with resistance to root lesion nematodes, crown rot and yellow spot

Sheedy, Jason ORCID: and Reen, Roslyn and Thompson, John ORCID: and Clewett, Tim (2011) Simultaneous selection of wheat lines with resistance to root lesion nematodes, crown rot and yellow spot. In: Wheat Breeding Assembly 2011, 24-26 Aug 2011, Perth, Australia.


Wheat is Australia’s largest grain crop with an annual value of $5 B with the northern grain region contributing around $883 M. Root lesion nematodes (RLN; Pratylenchus thornei [Pt] & P. neglectus [Pn]), crown rot (CR; Fusarium pseudograminearum) and yellow spot (YS; Pyrenophora tritici-repentis) are four economically important diseases that commonly occur in combination and cost the northern wheat industry $137 M annually. Therefore, there is a need to develop germplasm with combinations of disease resistance to minimise yield loss in these situations. To achieve this, the established screening methods for each disease were combined and where necessary modified (RLN: One plant grown in a pot with 330 g of pasteurised soil, inoculated with 1650 each of Pt and Pn at planting, soil harvested after 16 weeks and RLN extracted and counted; YS: spore suspension applied by aerosol sprayer to seedlings 7 days after planting [DAP] and rated on a 1 to 9 scale 18 DAP; CR: 10mL of liquid inoculum containing spores and hyphae applied at the plant base 7 DAP, leaf sheaths rated for percentage browning 21 DAP and 20mL/plant of Benomyl® [xx g/L] applied 3 times after rating), to allow testing of up to 4 diseases simultaneously. Initial experiments used up to 42 fixed cultivars covering resistant to susceptible reactions to each disease and a final experiment of 21 check cultivars and 132 F3’s from a CPI133872 x Janz double haploid (moderately resistant to Pt, Pn and YS) crossed to EGA Wylie (moderately resistant to CR) exposed to all 4 diseases simultaneously. YS reactions of fixed cultivars in multiple disease treatments were significantly correlated with the YS alone treatment (R2 up to 0.66***), as were CR treatments (R2 up to 0.48***). Similarly, the check cultivars in the F3 experiment were significantly correlated with long-term data for YS (R2 = 0.89***) and CR (R2 = 0.38**). RLN data has not been finalised but at least one F3 with resistance to all four diseases has been identified. These methods appear to have the potential to hasten the development of multiple-disease resistant parents, suitable for introduction into commercial breeding programs.

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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Speech)
Refereed: Yes
Item Status: Live Archive
Additional Information: Session 4: Biotic stress tolerance. Abstract only published. No evidence of copyright restrictions preventing deposit.
Faculty/School / Institute/Centre: No Faculty
Faculty/School / Institute/Centre: No Faculty
Date Deposited: 08 Sep 2016 06:27
Last Modified: 27 Sep 2019 04:36
Uncontrolled Keywords: root-legion nematodes; wheat production; Australia
Fields of Research (2008): 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Fields of Research (2020): 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3004 Crop and pasture production > 300409 Crop and pasture protection (incl. pests, diseases and weeds)

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