Common QTL and multi-purpose markers for the resistance of five synthetic hexaploids against two species of root lesion nematode

Khoo, Kelvin H. P. and Sheedy, Jason and Thompson, John and Hu, Chih-Fang and Vassos, Elysia and Fox, Rebecca and Chalmers, Ken and Mather, Diane E. (2013) Common QTL and multi-purpose markers for the resistance of five synthetic hexaploids against two species of root lesion nematode. In: Wheat Breeding Assembly 2013, 16-19 July 2013, Brisbane, Australia.

Abstract

The root lesion nematodes Pratylenchus thornei and P. neglectus are major constraints to cereal production. Among cultivated wheats, there are few good sources of resistance. Among synthetic hexaploid wheats, several lines had been discovered with partial resistance against both nematode species. Using genome-wide marker arrays (SNP and DArTTM) we mapped quantitative trait loci (QTL) for resistance from five partially resistant synthetics of diverse origin (CPI133872, CPI133842, CPI133859, TAMD870167/AUS18913 and Yallaroi/AUS18913). The results were very similar among resistance sources and across nematode species, with QTL detected on chromosome 2B and in two regions of chromosome 6D. To provide tools that wheat breeders can use to select for the synthetic-derived resistance, we added more SNP markers to the QTL regions and assayed those markers on a validation panel of wheat cultivars. This allowed us to identify sets of two or three markers per QTL region. For each set, all five synthetics share a haplotype that is rare or absent among the cultivated wheats in the panel. These markers can be rapidly assayed using KASPTM end-point genotyping and could be adapted to other genotyping technologies for application in wheat breeding.


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Item Type: Conference or Workshop Item (Commonwealth Reporting Category E) (Speech)
Refereed: Yes
Item Status: Live Archive
Additional Information: Abstract only published.
Faculty / Department / School: No Faculty
Date Deposited: 05 Sep 2016 03:52
Last Modified: 08 Mar 2017 05:12
Fields of Research : 07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
URI: http://eprints.usq.edu.au/id/eprint/29650

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