Enhancing the value of nitrogen from rapeseed meal for microbial oil production

Kiran, Esra Uckun and Salakkama, Apilak and Trzcinski, Antoine P. and Bakir, Ufuk and Webb, Colin (2012) Enhancing the value of nitrogen from rapeseed meal for microbial oil production. Enzyme and Microbial Technology , 50 (6-7). pp. 337-342. ISSN 0141-0229

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Abstract

Rapeseed meal, a major byproduct of biodiesel production, has been used as a low-cost raw material for the production of a generic microbial feedstock through a consolidated bioconversion process. Various strategies were tested for the production of a novel fermentation medium, rich in free amino nitrogen (FAN): commercial enzymes (CEs) (2.7 mg g−1 dry meal), liquid state fungal pre-treatment (LSF) using Aspergillus oryzae (4.6 mg g−1), liquid state fungal pre-treatment followed by fungal autolysis (LSFA) (9.13 mg g−1), liquid state pre-treatment using fungal enzymatic broth (EB) (2.1 mg g−1), but the best strategy was a solid state fungal pre-treatment followed by fungal autolysis (34.5 mg g−1).

The bioavailability of the nitrogen sources in the novel medium was confirmed in fed-batch bioreactor studies, in which 82.3 g dry cell L−1 of the oleaginous yeast Rhodosporidium toruloides Y4 was obtained with a lipid content of 48%. The dry cell weight obtained was higher than that obtained using conventional yeast extract, due to a higher total nitrogen content in the novel biomedium. The fatty acids obtained from the microbial oil were similar to those derived from rapeseed oil.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Accepted version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Engineering and Surveying - Department of Agricultural, Civil and Environmental Engineering
Date Deposited: 14 Jul 2016 05:31
Last Modified: 18 Jul 2018 04:31
Uncontrolled Keywords: rapeseed meal; fermentation; solid state fermentation; free amino nitrogen; Rhodosporidium toruloides; microbial oil
Fields of Research : 05 Environmental Sciences > 0502 Environmental Science and Management > 050205 Environmental Management
09 Engineering > 0907 Environmental Engineering > 090703 Environmental Technologies
Identification Number or DOI: 10.1016/j.enzmictec.2012.03.004
URI: http://eprints.usq.edu.au/id/eprint/28936

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