Denaturing gradient gel electrophoresis analysis of archaeal and bacterial populations in a submerged anaerobic membrane bioreactor treating landfill leachate at low temperatures

Trzcinski, Antoine P. and Stuckey, David C. (2012) Denaturing gradient gel electrophoresis analysis of archaeal and bacterial populations in a submerged anaerobic membrane bioreactor treating landfill leachate at low temperatures. Environmental Engineering Science , 29 (4). pp. 219-226. ISSN 1092-8758

[img]
Preview
Text (Accepted Version)
manuscriptEnvEngScience.pdf

Download (165Kb) | Preview

Abstract

This study investigated the evolution of archaeal and bacterial populations of two submerged anaerobic membrane bioreactors (SAMBRs) operating at a mean solids residence time of 30 (SAMBR30) and 300 days (SAMBR300) at mesophilic and psychrophilic temperatures. SAMBRs were fed with leachate produced in a hydrolytic reactor (HR) treating the organic fraction of municipal solid waste. The archaeal fingerprint using denaturing gradient gel electrophoresis showed different populations in the first and second stage of the two-stage anaerobic process. A build up of volatile fatty acids (VFAs) was observed at 20°C in SAMBR30; whereas in SAMBR300, the VFAs only built up at 10°C. The dominant bacterial species in the HR belonged to Prevotella and Thauera, whereas the dominant ones in SAMBR300 belonged to Sphingobacteriales, Anaerovorax, Spirochaetaceae, Hydrogenophaga, Ralstonia, Prevotella, and Smithella. Low bacterial diversity in SAMBR30 compared with SAMBR300 resulted in a persistently high soluble chemical oxygen demand (>2 g/L) in the bulk reactor due to an insufficient residence time for bacteria to carry out the degradation of recalcitrant chemical oxygen demand found in the leachate.


Statistics for USQ ePrint 28934
Statistics for this ePrint Item
Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Accepted version deposited din accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Engineering and Surveying - Department of Agricultural, Civil and Environmental Engineering
Date Deposited: 14 Jul 2016 04:51
Last Modified: 20 Jul 2018 02:31
Uncontrolled Keywords: anaerobic digestion; submerged anaerobic membrane bioreactor; denaturating gradient gel electrophoresis
Fields of Research : 05 Environmental Sciences > 0502 Environmental Science and Management > 050205 Environmental Management
09 Engineering > 0907 Environmental Engineering > 090703 Environmental Technologies
Identification Number or DOI: 10.1089/ees.2011.0003
URI: http://eprints.usq.edu.au/id/eprint/28934

Actions (login required)

View Item Archive Repository Staff Only