Comparative analysis of N-glycans in the ungerminated and germinated stages of Oryza sativa

Horiuchi, Risa and Hirotsu, Naoki and Miyanishi, Nobumitsu (2015) Comparative analysis of N-glycans in the ungerminated and germinated stages of Oryza sativa. Carbohydrate Research, 418. pp. 1-8. ISSN 0008-6215


All fundamental information such as signal transduction, metabolic control, infection, cell-to-cell signaling, and cell differentiation related to the growth of plants are preserved in germs. In preserving these information, glycans have a key role and are involved in the development and differentiation of organisms. Glycans which exist in rice germ are expected to have an important role in germination. In this study, we performed structural and correlation analysis of the N-glycans in rice germ before and after germination. Our results confirmed that the N-glycans in the ungerminated stage of the rice germ had low number of N-glycans consisting only of six kinds especially with highmannose and paucimannose type N-glycans being 16.0% and 76.7%, respectively. On the other hand, after 48 hours germinated germ stage, there was an increase in the complex type N-glycans with the appearance of Lewis a structure, the most complex type and a decrease in paucimannose types. These results suggest that at least six kinds of N-glycans are utilized for long time preservation of rice seed, while the diversification of most complex types of N-glycans is produced an environment dependent for shoot formation of rice.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Files associated with this item cannot be displayed due to copyright restrictions.
Faculty / Department / School: No Faculty
Date Deposited: 14 Jun 2016 03:38
Last Modified: 20 Mar 2017 05:10
Uncontrolled Keywords: Oryza sativa; N-Glycans; germination; Lewis a
Fields of Research : 06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060101 Analytical Biochemistry
06 Biological Sciences > 0607 Plant Biology > 060703 Plant Developmental and Reproductive Biology
Identification Number or DOI: 10.1016/j.carres.2015.09.008

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