Preparation of C-reactive protein latex reagent of human serum

Alshalchi, Sahar A. and Mahdi, Layla K. and Sabar, Muna (2006) Preparation of C-reactive protein latex reagent of human serum. Iraqi Journal of Biotechnology, 5 (1). pp. 112-122. ISSN 1815-4794

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Abstract

C-reactive protein (CRP) is an acute phase reactant the concentration of which increases significantly following tissue injury or inflammation. It is present in the albumin fraction of the serum of patients and is antigenic upon injection into rabbits. We report here the development of a local latex reagent coated with rabbit anti-CRP. For this purpose CRP was isolated from pooled sera of patients and injected into rabbits. CRP rabbit antiserum was purified by
using ammonium sulfate precipitation. Followed by passing on ion exchange chromatography (Diethylaminoethyl 'DEAE' cellulose). Then, latex particles were coated with purified antisera. The prepared CRP latex reagent agglutination was observed upon the addition of patients' sera and compared with that of normal sera. In addition, it was evaluated against a standard CRP latex reagent. The prepared reagent gave positive results (100%) with patients' sera and the positive control of the standard kit. While, the negative control of the standard kit and 30% of the normal sera gave negative results.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 27 Feb 2018 06:53
Last Modified: 27 Feb 2018 06:53
Uncontrolled Keywords: CRP, latex agglutination
Fields of Research : 11 Medical and Health Sciences > 1103 Clinical Sciences > 110303 Clinical Microbiology
06 Biological Sciences > 0605 Microbiology > 060501 Bacteriology
11 Medical and Health Sciences > 1103 Clinical Sciences > 110309 Infectious Diseases
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences
URI: http://eprints.usq.edu.au/id/eprint/28831

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