Molecular identification of the primary root fungal endophytes of Dipodium hamiltonianum (Orchidaceae)

Dearnaley, John and Le Brocque, Andrew F. (2006) Molecular identification of the primary root fungal endophytes of Dipodium hamiltonianum (Orchidaceae). Australian Journal of Botany, 54 (5). pp. 487-491. ISSN 0067-1924

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Abstract

[Abstract]: We have identified the primary root fungal endophytes of Dipodium hamiltonianum F.M.Bailey, a species
of orchid endangered in Victoria and uncommon in New South Wales and Queensland. GenomicDNAwas extracted
from whole colonised root portions from four orchid individuals and PCR amplified with ITS1F and ITS4 primers.
Cloning and sequencing of the main amplicons produced from the PCR analysis revealed that the primary root
fungal endophytes were Gymnomyces and Russula spp., both members of the Russulaceae. The implications of
these findings are discussed in terms of conservation of the orchid species.

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Item Type:	Article (Commonwealth Reporting Category C)
Refereed:	Yes
Item Status:	Live Archive
Additional Information:	Deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School:	Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited:	11 Oct 2007 01:15
Last Modified:	02 Jul 2013 22:47
Uncontrolled Keywords:	molecular identification, root fungal endophytes, Dipodium hamiltonianum, Orchidaceae, orchids
Fields of Research :	06 Biological Sciences > 0607 Plant Biology > 060702 Plant Cell and Molecular Biology 06 Biological Sciences > 0605 Microbiology > 060505 Mycology 06 Biological Sciences > 0607 Plant Biology > 060799 Plant Biology not elsewhere classified
Identification Number or DOI:	doi: 10.1071/BT05149
URI:	http://eprints.usq.edu.au/id/eprint/2883

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