Central role of manganese in regulation of stress responses, physiology, and metabolism in streptococcus pneumoniae

Ogunniyi, Abiodun D. and Mahdi, Layla K. and Jennings, Michael P. and McEwan, Alastair G. and McDevitt, Christopher A. and Van der Hoek, Mark B. and Bagley, Christopher J. and Hoffmann, Peter and Gould, Katherine A. and Paton, James C. (2010) Central role of manganese in regulation of stress responses, physiology, and metabolism in streptococcus pneumoniae. Journal of Bacteriology, 192 (17). pp. 4489-4497. ISSN 0021-9193

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The importance of Mn(2+) for pneumococcal physiology and virulence has been studied extensively. However, the specific cellular role(s) for which Mn(2+) is required are yet to be fully elucidated. Here, we analyzed the effect of Mn(2+) limitation on the transcriptome and proteome of Streptococcus pneumoniae D39. This was carried out by comparing a deletion mutant lacking the solute binding protein of the high-affinity Mn(2+) transporter, pneumococcal surface antigen A (PsaA), with its isogenic wild-type counterpart. We provide clear evidence for the Mn(2+)-dependent regulation of the expression of oxidative-stress-response enzymes SpxB and Mn(2+)-SodA and virulence-associated genes pcpA and prtA. We also demonstrate the upregulation of at least one oxidative- and nitrosative-stress-response gene cluster, comprising adhC, nmlR, and czcD, in response to Mn(2+) stress. A significant increase in 6-phosphogluconate dehydrogenase activity in the psaA mutant grown under Mn(2+) replete conditions and upregulation of an oligopeptide ABC permease (AppDCBA) were also observed. Together, the results of transcriptomic and proteomic analyses provided evidence for Mn(2+) having a central role in activating or stimulating enzymes involved in central carbon and general metabolism. Our results also highlight the importance of high-affinity Mn(2+) transport by PsaA in pneumococcal competence, physiology, and metabolism and elucidate mechanisms underlying the response to Mn(2+) stress.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Access to published version in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 27 Feb 2018 01:59
Last Modified: 27 Feb 2018 02:19
Uncontrolled Keywords: ABC permease; bacterial antigen; gene product; manganese; oligopeptide; permease; phosphogluconate dehydrogenase; pneumococcal surface antigen A; protein soda; protein SpxB; proteome; transcriptome; unclassified drug; adhesion; bacterial protein; lipoprotein; manganese; proteome; PsaA protein; Streptococcus
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060501 Bacteriology
11 Medical and Health Sciences > 1108 Medical Microbiology > 110801 Medical Bacteriology
06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences
Funding Details:
Identification Number or DOI: 10.1128/JB.00064-10
URI: http://eprints.usq.edu.au/id/eprint/28820

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