A transcription factor contributes to pathogenesis and virulence in Streptococcus pneumoniae

Mahdi, Layla K. and Ebrahimie, Esmaeil and Adelson, David L. and Paton, James C. and Ogunniyi, Abiodun D. (2013) A transcription factor contributes to pathogenesis and virulence in Streptococcus pneumoniae. PLoS One, 8 (8). e70862-e70871.

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Abstract

To date, the role of transcription factors (TFs) in the progression of disease for many pathogens is yet to be studied in detail. This is probably due to transient, and generally low expression levels of TFs, which are the central components controlling the expression of many genes during the course of infection. However, a small change in the expression or specificity of a TF can radically alter gene expression. In this study, we combined a number of quality-based selection strategies including structural prediction of modulated genes, gene ontology and network analysis, to predict the regulatory mechanisms underlying pathogenesis of Streptococcus pneumoniae (the pneumococcus). We have identified two TFs (SP_0676 and SP_0927 [SmrC]) that might control tissue-specific gene expression during pneumococcal translocation from the nasopharynx to lungs, to blood and then to brain of mice. Targeted mutagenesis and mouse models of infection confirmed the role of SP_0927 in pathogenesis and virulence, and suggests that SP_0676 might be essential to pneumococcal viability. These findings provide fundamental new insights into virulence gene expression and regulation during pathogenesis.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version made available in accordance with Creative Commons Attribution License 4.0.
Faculty / Department / School: Historic - Faculty of Engineering and Surveying - No Department
Date Deposited: 03 Aug 2016 05:49
Last Modified: 27 Mar 2017 05:17
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060503 Microbial Genetics
06 Biological Sciences > 0605 Microbiology > 060501 Bacteriology
11 Medical and Health Sciences > 1108 Medical Microbiology > 110801 Medical Bacteriology
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
E Expanding Knowledge > 97 Expanding Knowledge > 970111 Expanding Knowledge in the Medical and Health Sciences
Funding Details:
Identification Number or DOI: 10.1371/journal.pone.0070862
URI: http://eprints.usq.edu.au/id/eprint/28816

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