Effects of HIF-1α on ERRα/γ protein expression in mouse skeletal muscle

Zhang, Ying and Ji, Weixiu and Zhang, Lianfeng and Liu, Sixue and Liu, Gang and Wang, Jianxiong (2015) Effects of HIF-1α on ERRα/γ protein expression in mouse skeletal muscle. Edorium Journal of Biomedical Technology, 1. pp. 4-10.

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Aims: HIF-1α plays an important role in the adaptive responses to hypoxia. The ERRα and γ are crucial regulators of energy metabolism in skeletal muscle. The aim of the present study was to generate the inducible HIF-1α transgenic mice and examine the effects of different HIF-1α
protein expression levels on ERRα/γ in mouse skeletal muscle. Methods: We generated the HIF-1α high-expression transgenic mice (HT)and HIF-1α low-expression transgenic mice (LT),and then compared the expressions of ERRα/γ
and its target genes in skeletal muscles of three kinds of mice: HT, LT, and non-transgenic mice (NT).Results: The results showed that (1) the double positive mice from the founder of 3# and 68# showed an obvious expression of HIF-
1α induced by tamoxifen and both of them were maintained to the further research as HT and LT mice, respectively; and (2) the nucleoprotein expressions of ERRα/γ and the mRNA levels of the ERRα/γ target genes: MCAD, PPARα, NRF1
and PDK4 were higher in the LT mice than the values in the NT, but only the mRNA levels of MCAD and PPARα were significantly higher. The HT mice showed significantly lower ERRα protein content than that of the NT mice. Conclusion: Our study was the first to report the generation of the inducible HIF-1α transgenic mice and effects of HIF-1α on ERRα/γ protein expression in mouse
skeletal muscle in vivo. These data demonstrate that the low HIF-1α protein expression may associate with an up-regulation of ERRα/γ and their target genes in skeletal muscles, while the aggravated HIF-1α protein expression would reduce the effects.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Paper made available under the Creative Commons Attribution 4.0 International License.
Faculty/School / Institute/Centre: Current - Faculty of Health, Engineering and Sciences - School of Health and Wellbeing
Date Deposited: 01 Apr 2016 05:50
Last Modified: 02 Sep 2016 05:16
Uncontrolled Keywords: ERRα/γ, HIF-1α, skeletal muscle, target genes, transgenic mouse
Fields of Research : 11 Medical and Health Sciences > 1106 Human Movement and Sports Science > 110602 Exercise Physiology
06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060111 Signal Transduction
URI: http://eprints.usq.edu.au/id/eprint/28613

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