Lee, Jaslyn Jie Lin and Chen, Liwei and Shi, Jiahua and Trzcinski, Antoine and Chen, Wei-Ning (2014) Metabolomic profiling of Rhodosporidium toruloides grown on glycerol for carotenoid production during different growth phases. Journal of Agricultural and Food Chemistry, 62 (41). pp. 10203-10209. ISSN 0021-8561
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Abstract
Carotenoid production from three strains of Rhodosporidium toruloides grown on glycerol was studied. A timedependent metabolomics approach was used to understand its metabolism on glycerol and mechanism for carotenoid production in three strains during different growth phases (1, 4, 7, and 12 days). Strain CBS 5490 was the highest carotenoid producer (28.5 mg/L) and had a unique metabolic profile. In this strain, metabolites belonging to the TCA cycle and amino acids were produced in lower amounts, as compared to the other strains. On the other hand, it produced the highest amounts of carotenoid and fatty acid metabolites. This indicated that the lower production of the TCA cycle and amino acid metabolites promoted energy and metabolic flux toward the carotenoid and fatty acid synthesis metabolic pathways. This study shows that metabolomic profiling is a useful tool to gain insight into the metabolic pathways in the cell and to shed light on the different molecular mechanisms between strains.
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| Item Type: | Article (Commonwealth Reporting Category C) |
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| Refereed: | Yes |
| Item Status: | Live Archive |
| Additional Information: | Accepted version deposited in accordance with the copyright policy of the publisher. |
| Faculty / Department / School: | Current - Faculty of Health, Engineering and Sciences - School of Civil Engineering and Surveying |
| Date Deposited: | 14 Jul 2016 03:37 |
| Last Modified: | 18 Jul 2018 04:21 |
| Uncontrolled Keywords: | metabolomics; glycerol; R. toruloides; carotenoids; GC-MS |
| Fields of Research : | 05 Environmental Sciences > 0502 Environmental Science and Management > 050205 Environmental Management |
| Identification Number or DOI: | 10.1021/jf502987q |
| URI: | http://eprints.usq.edu.au/id/eprint/28562 |
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