Effects of different exercise durations on Keap1-Nrf2-ARE pathway activation in mouse skeletal muscle

Li, Tieying and He, Sheyi and Liu, Sixue and Kong, Zhenxing and Wang, Jianxiong and Zhang, Ying (2015) Effects of different exercise durations on Keap1-Nrf2-ARE pathway activation in mouse skeletal muscle. Free Radical Research , 49 (10). pp. 1269-1274. ISSN 1071-5762


The purpose of this study was to investigate the effects of acute exercise stress on the nuclear factor-erythroid2 p45-related factor 2 (Nrf2)/antioxidant response element (ARE) transactivation, Kelch-like ECH-associated protein 1(Keap1) cytosolic protein and Nrf2 nucleoprotein expressions, Nrf2 target genes mRNA expressions, and GSH/GSSG ratio level; with a particular focus on the changes of Keap1-Nrf2-ARE pathway activation following different durations of exercise. Wild-type mice (C57BL/6J, two months old) were separated into one-hour and six-hour treadmill running groups, as well as a non-exercise control group (n=10 in each group). Measurements of Nrf2/ARE transactivation, Nrf2 nucleoprotein expressions, Keap1 cytosolic protein expression, Nrf2 target genes' mRNA expressions (superoxide dismutase-1 (SOD1), superoxide dismutase-2 (SOD2), γ-glutamyl cysteine ligase-modulatory (GCLm), γ-glutamylcysteine ligase-catalytic (GCLc), glutathione reductase (GR), glutathione peroxydase-1 (Gpx1), catalase (CAT) and hemoxygenase-1 (Ho-1)), and glutathione redox (GSH/GSSG) ratio were carried out immediately after exercise. The results showed significant increases in Keap1-Nrf2-ARE pathway activation and the mRNA expressions of six measured enzymes in skeletal muscle after six hours of exercise; while in the one-hour exercise group, there was no change in Keap1-Nrf2-ARE pathway activation and only two enzymes' mRNA expressions were increased. It is suggested that the changes in Keap1-Nrf2-ARE pathway activation and its target genes' mRNA expressions were dependent on the exercise duration, with longer duration associated with higher responses.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Permanent restricted access to ArticleFirst version according to the publisher's copyright policy.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Health and Wellbeing
Date Deposited: 10 Nov 2015 01:47
Last Modified: 20 Sep 2017 04:12
Uncontrolled Keywords: Nrf2 transactivation, oxidative stress, acute exercise, antioxidant enzyme
Fields of Research : 11 Medical and Health Sciences > 1106 Human Movement and Sports Science > 110602 Exercise Physiology
Identification Number or DOI: 10.3109/10715762.2015.1066784
URI: http://eprints.usq.edu.au/id/eprint/27746

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