The potential for reductive mobilization of arsenic [As(V) to As(III)] by OSBH2 (Pseudomonas stutzeri) and OSBH5 (Bacillus cereus) in an oil-contaminated site

Maity, Jyoti Prakash and Kar, Sandeep and Liu, Jiann-Hong and Jean, Jiin-Shuh and Chen, Chien-Yen and Bundschuh, Jochen and Santra, Subhas Chandra and Liu, Chia-Chuan (2011) The potential for reductive mobilization of arsenic [As(V) to As(III)] by OSBH2 (Pseudomonas stutzeri) and OSBH5 (Bacillus cereus) in an oil-contaminated site. Journal of Environmental Science and Health: Part A Toxic Hazardous Substances and Environmental Engineering, 46 (11). pp. 1239-1246. ISSN 1093-4529

Abstract

Microbial reduction of arsenate [As(V)] plays an important role in arsenic (As) mobilization in aqueous environments. In this study, we investigated reduction of arsenate by different bacterial isolates such as OSBH1 (GU329913), OSBH2 (GU329914), OSBH3 (GU329915), OSBH4 (GU329916) and OSBH5 (GU329917), isolated from the oil sludge of a sewage treatment plant operated by the China Petroleum Refinery Company in Kaohsiung, southern Taiwan. Bacterial strains of pure culture were identified by 16S rRNA analysis (≥99 % nucleotide similarity). Morphological and 16S rRNA analysis show that the isolate OSBH1 is similar to E. coli, OSBH2 is similar to P. stutzeri, OSBH3 is similar to P. putida, OSBH4 is similar to P. aeruginosa, and OSBH5 is similar to B. Cereus. The As(V) was transformed to As(III) in the presence of isolates OSBH2 and OSBH5 by a detoxification process. The potential reduction rates of As(V) were higher in the presence of isolate OSBH5 compared to the isolate OSBH2. The microbial growth (cell/mL) of isolate OSBH5 was significantly higher in culture medium compared to OSBH2. The bacterial isolates such as OSBH1, OSBH3 and OSBH4 were found to be incapable of transforming the As(V). It is concluded that the activity of the oil-degrading bacterial isolates described in this work contributes to the mobilization of As in the more toxic As(III) form that affects biotic life.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published Version restricted in Accordance with publisher copyright policy (T&F)
Faculty / Department / School: Historic - Faculty of Engineering and Surveying - No Department
Date Deposited: 05 Sep 2014 02:06
Last Modified: 31 Jan 2017 00:59
Uncontrolled Keywords: arsenic mobilization, biogeochemistry, oil contamination, aquifer, Taiwan
Fields of Research : 05 Environmental Sciences > 0502 Environmental Science and Management > 050204 Environmental Impact Assessment
05 Environmental Sciences > 0502 Environmental Science and Management > 050207 Environmental Rehabilitation (excl. Bioremediation)
05 Environmental Sciences > 0503 Soil Sciences > 050399 Soil Sciences not elsewhere classified
05 Environmental Sciences > 0502 Environmental Science and Management > 050205 Environmental Management
05 Environmental Sciences > 0502 Environmental Science and Management > 050206 Environmental Monitoring
Socio-Economic Objective: D Environment > 96 Environment > 9612 Rehabilitation of Degraded Environments > 961208 Rehabilitation of Degraded Urban and Industrial Environments
D Environment > 96 Environment > 9609 Land and Water Management > 960908 Mining Land and Water Management
D Environment > 96 Environment > 9609 Land and Water Management > 960999 Land and Water Management of Environments not elsewhere classified
Identification Number or DOI: 10.1080/10934529.2011.598802
URI: http://eprints.usq.edu.au/id/eprint/25819

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