New insight into photosynthetic acclimation to elevated CO2: the role of leaf nitrogen and ribulose-1,5-bisphosphate carboxylase/oxygenase content in rice leaves

Seneweera, Saman and Makino, Amane and Hirotsu, Naoki and Norton, Robert and Suzuki, Yuji (2011) New insight into photosynthetic acclimation to elevated CO2: the role of leaf nitrogen and ribulose-1,5-bisphosphate carboxylase/oxygenase content in rice leaves. Environmental and Experimental Botany, 71 (2). pp. 128-136. ISSN 0098-8472

Abstract

We tested the hypothesis that photosynthetic (A) acclimation to elevated CO2 partial pressure (p[CO2]) is associated with the inhibition of protein synthesis, inhibition of nitrogen (N) partitioning into the leaf blade and/or accelerated leaf senescence in rice (Oryza sativa L. cv. Notohikari). Plants were grown for 70 days hydroponically in artificially illuminated growth chambers at a p[CO2] of either 39 or 100Pa at N 2mM. Leaf A, Vc.max, Jmax, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, E.C.4.1.1.39), mRNA for associated genes rbcS and rbcL, total N and carbohydrate concentrations in leaves at different positions in the canopy were measured. Spatial allocation of N and Rubisco synthesis of expanding leaf blade was also measured from the leaf ligule to tip of the leaf blade. Growth at elevated p[CO2] suppressed light saturated A, Vc.max and Jmax in leaf blades at all positions in the canopy. The suppression of A was 15% for the upper leaf blades compared to 37% in the lower leaf blades. Similar reductions in the amount of Rubisco, Chlorophyll, and total N were observed in the leaves of the plants grown in 100 p[CO2] compared to the 39 p[CO2]. Sucrose and starch concentration concentrations increased at elevated p[CO2] but we found no relationship between A, Rubisco or the amount of transcript abundance of rbcS and rbcL. Elevated p[CO2] substantially reduced N allocation into expanding leaf blades and this was well correlated with Rubisco synthesis. These results suggest that A acclimation to elevated p[CO2] occurs during all phases of the leaf development, is initiated during the cell maturation process and linked with spatial N allocation into the leaf blade. In addition, elevated p[CO2] accelerated lower leaf blade senescence which compounded the effect on A acclimation.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2010 Elsevier B.V. Permanent restricted access to published version in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 01 Jul 2014 23:16
Last Modified: 09 Feb 2017 05:11
Uncontrolled Keywords: carbohydrates; elevated p[CO2]; nitrogen; photosynthesis; photosynthetic acclimation; Ribulose-1,5-bisphosphate carboxylase/oxygenase; rice; Oryza sativa L. cv. Notohikari
Fields of Research : 10 Technology > 1001 Agricultural Biotechnology > 100103 Agricultural Molecular Engineering of Nucleic Acids and Proteins
06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
03 Chemical Sciences > 0305 Organic Chemistry > 030503 Organic Chemical Synthesis
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Identification Number or DOI: 10.1016/j.envexpbot.2010.11.002
URI: http://eprints.usq.edu.au/id/eprint/25406

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