Impact of microencapsulated peptidase (Aspergillus oryzae) on cheddar cheese proteolysis and its biologically active peptide profile

Seneweera, Saman and Kailasapathy, Kaila (2011) Impact of microencapsulated peptidase (Aspergillus oryzae) on cheddar cheese proteolysis and its biologically active peptide profile. Protein and Peptide Letters, 18 (7). pp. 741-746. ISSN 0929-8665


We investigated the delivery of calcium-alginate encapsulated peptidase (Flavourzyme®, Aspergillus oryzae) on proteolysis of Cheddar cheese. Physical and chemical characteristics such as moisture, pH and fat content were measured, and no differences were found between control and experimental cheese at day 0. SDS-PAGE analysis clearly showed that proteolysis of β and k casein was significantly accelerated after three months of maturity in the experimental cheese. A large number of low molecular weight peptides were found in the water soluble fraction of the experimental cheeses and some of these peptides were new. N-terminal amino acid sequence analysis identified these as P 1, Leu-Thu- Glu; P 3, Asp-Val-Pro-Ser-Glu and relatively abundant stable peptides P 2, P 4, Arg-Pro-Lys-His-Pro-Ile; P 5, Arg-Pro-Lys- His-Pro-Ile-Lys and P 6. These peptides were mainly originated from βs1-CN and β-CN. Four of the identified peptides (P 1, P 2, P 3 and P 4) are known to be biologically active and P 1 and P 3 were only present in experimental cheese suggesting that experimental cheese has improved health benefits.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2011 Bentham Science Publishers Ltd. Published version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 01 Jul 2014 22:50
Last Modified: 09 Feb 2017 02:01
Uncontrolled Keywords: bioactive peptides; microencapsulation proteolysis; casein; cheese ripening
Fields of Research : 06 Biological Sciences > 0601 Biochemistry and Cell Biology > 060107 Enzymes
03 Chemical Sciences > 0304 Medicinal and Biomolecular Chemistry > 030406 Proteins and Peptides
11 Medical and Health Sciences > 1101 Medical Biochemistry and Metabolomics > 110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970103 Expanding Knowledge in the Chemical Sciences
Identification Number or DOI: 10.2174/092986611795446076

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