A single backcross effectively eliminates agronomic and quality alterations caused by somaclonal variation in transgenic barley

Bregitzer, P. and Dahleen, Lynn S. and Neate, Stephen and Schwarz, Paul and Manoharan, Muthusamy (2008) A single backcross effectively eliminates agronomic and quality alterations caused by somaclonal variation in transgenic barley. Crop Science, 48 (2). pp. 471-479. ISSN 0011-183X

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Abstract

Transgenic crops have proven commercial utility but are created using processes known to produce undesirable variability known as somaclonal variation. This reduces the utility of transgenic germplasm to the plant breeder and complicates assessments of transgene-encoded phenotypes. Backcrossing transgenes into a wild-type genome is one solution, but producing near-isogenic lines requires a lengthy and resource-intensive process of multiple crosses. However, an abbreviated breeding scheme involving a single backcross to the wild-type parent used to produce a transgenic line, which would replace 75% of the variant alleles, should produce transgenic lines with improved performance. Comparisons were made of 'Conlon' barley (Hordeum vulgare L.), primary transgenic lines derived from Conlon, and lines derived from single backcrosses of primary transgenic lines to Conlon. The primary transgenic lines were different from Conlon for many agronomic and malting characteristics. Most of the backcross-derived lines did not differ significantly from Conlon for most agronomic characteristics. The backcross-derived lines were also similar to Conlon for malting quality traits but showed more differences than for agronomic characteristics. Differences between lines encoding TRI101 versus lines encoding PDR5 suggested that PDR5 insertion or expression may have affected malting quality. It is concluded that a single backcross is an effective, rapid, and inexpensive method for creating superior transgenic lines.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2008 Crop Science Society of America. Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. Published version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 13 Jun 2014 01:26
Last Modified: 18 Aug 2014 06:25
Uncontrolled Keywords: Hordeum vulgare subsp vulgare; transgenesis; cross breeding; disease resistance; somaclonal variation; transgenic plants; backcrossing; germplasm; phenotype; genome; isogenic lines; alleles; malting quality
Fields of Research : 06 Biological Sciences > 0604 Genetics > 060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics)
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
10 Technology > 1001 Agricultural Biotechnology > 100109 Transgenesis
Socio-Economic Objective: B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820501 Barley
Identification Number or DOI: 10.2135/cropsci2007.06.0370
URI: http://eprints.usq.edu.au/id/eprint/25294

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