Comparison of ELISA for Fusarium, visual screening, and deoxynivalenol analysis of Fusarium head blight for barley field nurseries

Hill, N. S. and Neate, S. M. and Cooper, B. and Horsley, R. and Schwarz, P. and Dahleen, L. S. and Smith, K. P. and O'Donnell, K. and Reeves, J. (2008) Comparison of ELISA for Fusarium, visual screening, and deoxynivalenol analysis of Fusarium head blight for barley field nurseries. Crop Science, 48 (4). pp. 1389-1398. ISSN 0011-183X

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Abstract

Breeding for resistance to Fusarium head blight {FHB; caused by Fusarium graminearum Schwabe [teleomorph Gibberella zeae (Schwein) Petch]} is complicated because there is no accurate method for quantifying the disease organism. Most breeders rely on visual scoring for FHB and deoxynivalenol (DON) analysis to assess disease severity, but both DON and visual scoring are subject to error. The objective of this study was to compare a Fusarium-specific quantifiable enzyme-linked immunosorbent assay (ELISA) to DON and visual assessment of FHB. A doubled-haploid mapping population was grown in two environments and breeding lines in the North American Barley Scab Evaluation Nursery (NABSEN) were grown at four locations. Both experiments used a randomized complete block design and were analyzed for Fusarium by ELISA, DON, and visually scored for FHB. ELISA values for the doubled-haploid lines were consistent over years, and lines that were low in ELISA were also low in DON. Broad sense heritability was greater for ELISA (0.48) than DON (0.19) or visual scores of disease (0.29) in the NABSEN study. Numbers of locations and replications necessary to screen for disease were calculated for ELISA, DON, and FHB. ELISA required one-third to one-fourth as many replications and locations as did DON or FHB. Temperature and osmotic potential had little effect on mycelial antigen used for ELISA in vitro, but both affected DON. ELISA is a practical alternative to combined visual scores and DON analysis for breeders interested in improving FHB resistance.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Copyright © 2008 Crop Science Society of America. Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. Published version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 13 Jun 2014 00:15
Last Modified: 18 Aug 2014 06:26
Uncontrolled Keywords: Fusarium; Gibberella zeae; Hordeum; barley; scab diseases; enzyme-linked immunosorbent assay; disease diagnosis; diagnostic techniques; deoxynivalenol; breeding lines; disease resistance; genetic resistance; chromosome mapping
Fields of Research : 06 Biological Sciences > 0604 Genetics > 060407 Genome Structure and Regulation
06 Biological Sciences > 0605 Microbiology > 060505 Mycology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: 10.2135/cropsci2007.05.0266
URI: http://eprints.usq.edu.au/id/eprint/25293

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