Depth-resolved abundance and diversity of arsenite-oxidizing bacteria in the groundwater of Beimen, a blackfoot disease endemic area of southwestern Taiwan

Das, Suvendu and Kar, Sandeep and Jean, Jiin-Shuh and Rathod, Jagat and Chakraborty, Sukalyan and Liu, Hsiao-Sheng and Bundschuh, Jochen (2013) Depth-resolved abundance and diversity of arsenite-oxidizing bacteria in the groundwater of Beimen, a blackfoot disease endemic area of southwestern Taiwan. Water Research, 47 (19). pp. 6983-6991. ISSN 0043-1354

Abstract

The role of arsenite oxidizers in natural attenuation of arsenic pollution necessitates studies on their abundance and diversity in arsenic-contaminated aquifers. In this study, most probable number-polymerase chain reaction (MPN-PCR) and denaturing gradient gel electrophoresis (DGGE) was applied to monitor depth-wise abundance and diversity of aerobic arsenite oxidizers in arsenic-enriched groundwater of Beimen, southwestern Taiwan. The results revealed that the abundance of arsenite oxidizers ranged from 0.04 to 0.22, and the lowest ratio was observed in the most arsenic-enriched and comparatively more reduced groundwater (depth 200m) of Beimen 1. The highest ratio was observed in the less arsenic-enriched and less reduced groundwater (depth 60m) of Beimen 2B. DGGE profiles showed a shift in diversity of arsenite oxidizers, consisting of members of the Betaproteobacteria (61%), Alphaproteobacteria (28%) and Gammaproteobacteria (11%), depending on mainly arsenic concentration and redox level in groundwater. Groundwater with the lowest arsenic and highest dissolved oxygen at Beimen 2B harbored 78% of the arsenite oxidizers communities, while groundwater with the highest arsenic and lowest dissolved oxygen at Beimen 1 and Beimen-Jinhu harbored 17 and 22% of arsenite oxidizers communities, respectively. Pseudomonas sp. was found only in groundwater containing high arsenic at Beimen 1 and Beimen-Jinhu, while arsenite oxidizers belonging to Alpha- and Betaproteobacteria were dominated in groundwater containing low arsenic.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Published version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences
Date Deposited: 21 May 2014 23:24
Last Modified: 17 Jul 2014 05:21
Uncontrolled Keywords: arsenite oxidizing bacteria; groundwater arsenic; MPN-PCR; PCR-DGGE
Fields of Research : 10 Technology > 1002 Environmental Biotechnology > 100204 Environmental Biotechnology Diagnostics (incl. Biosensors)
07 Agricultural and Veterinary Sciences > 0799 Other Agricultural and Veterinary Sciences > 079901 Agricultural Hydrology (Drainage, Flooding, Irrigation, Quality, etc.)
09 Engineering > 0905 Civil Engineering > 090509 Water Resources Engineering
Socio-Economic Objective: D Environment > 96 Environment > 9611 Physical and Chemical Conditions of Water > 961103 Physical and Chemical Conditions of Water in Fresh, Ground and Surface Water Environments (excl. Urban and Industrial Use)
Identification Number or DOI: 10.1016/j.watres.2013.07.049
URI: http://eprints.usq.edu.au/id/eprint/25230

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