Genome-wide association mapping of Fusarium head blight resistance in contemporary barley breeding germplasm

Massman, J. and Cooper, B. and Horsley, R. and Neate, S. and Dill-Macky, R. and Chao, S. and Dong, Y. and Schwarz, P. and Muehlbauer, G. J. and Smith, K. P. (2011) Genome-wide association mapping of Fusarium head blight resistance in contemporary barley breeding germplasm. Molecular Breeding, 27 (4). pp. 439-454. ISSN 1380-3743

Abstract

Utilization of quantitative trait loci (QTL) identified in bi-parental mapping populations has had limited success for improving complex quantitative traits with low to moderate heritability. Association mapping in contemporary breeding germplasm may lead to more effective marker strategies for crop improvement. To test this approach, we conducted association mapping of two complex traits with moderate heritability; Fusarium head blight (FHB) severity and the grain concentration of mycotoxin associated with disease, deoxynivalenol (DON). To map FHB resistance in barley, 768 breeding lines were evaluated in 2006 and 2007 in four locations. All lines were genotyped with 1,536 SNP markers and QTL were mapped using a mixed model that accounts for relatedness among lines. Average linkage disequilibrium within the breeding germplasm extended beyond 4 cM. Four QTL were identified for FHB severity and eight QTL were identified for the DON concentration in two independent sets of breeding lines. The QTL effects were small, explaining 1–3% of the phenotypic variation, as might be expected for complex polygenic traits. We show that using breeding germplasm to map QTL can complement bi-parental mapping studies by providing independent validation, mapping QTL with more precision, resolving questions of linkage and pleiotropy, and identifying genetic markers that can be applied immediately in crop improvement.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2010 Springer Science+Business Media B.V. Published version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 12 Jun 2014 06:22
Last Modified: 09 Feb 2017 05:09
Uncontrolled Keywords: disease resistance; Fusarium head blight; Deoxynivalenol quantitative trait loci; association mapping; linkage disequilibrium; barley
Fields of Research : 06 Biological Sciences > 0604 Genetics > 060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics)
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
Socio-Economic Objective: B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820501 Barley
Identification Number or DOI: 10.1007/s11032-010-9442-0
URI: http://eprints.usq.edu.au/id/eprint/25191

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