Genetic structure of Cochliobolus sativus populations sampled from roots and leaves of barley and wheat in North Dakota

Gyawali, Sanjaya and Neate, Stephen M. and Adhikari, Tika B. and Puri, Krishna D. and Burlakoti, Rishi R. and Zhong, Shaobin (2012) Genetic structure of Cochliobolus sativus populations sampled from roots and leaves of barley and wheat in North Dakota. Journal of Phytopathology, 160 (11-12). pp. 637-646. ISSN 0931-1785


Common root rot (CRR) and spot blotch, caused by Cochliobolus sativus (Ito and Kurib.) Drechsl. ex Dast., are important diseases of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) worldwide. However, the population biology of C. sativus is still poorly understood. In this study, the genetic structure of three C. sativus populations, consisting of isolates sampled respectively from barley leaves (BL), barley roots (BR)and wheat roots (WR) in North Dakota, was analysed with amplified fragment length polymorphism (AFLP) markers. A total of 127 AFLP loci were generated among 208 C. sativus isolates analysed with three primer combinations.Gene diversity (H = 0.277–0.335) were high in all three populations. Genetic variation among C. sativus individuals within population accounted for 74%, whereas 26% of the genetic variation was explained among populations. Genetic differentiation was high (ØPT = 0.261, corrected G'st= 0.39), whereas gene flow (Nm) ranged from 1.27 to 1.56 among the three populations analysed. The multilocus linkage disequilibrium (LD) (rd= 0.076–0.117) was moderate in C sativus populations. Cluster analyses indicate that C. sativus populations differentiated according to the hosts (barley and wheat) and tissues (root and leaf) although generalists also exist in North Dakota. Crop breeding may benefit from combining genes for resistance against both specialists and generalists of C. sativus.

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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2012 Blackwell Verlag GmbH. Published version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 12 Jun 2014 05:17
Last Modified: 06 Mar 2018 04:41
Uncontrolled Keywords: Cochliobolus sativus; amplified fragment length polymorphism; genetic differentiation; common root rot; spot blotch; barley; wheat
Fields of Research : 06 Biological Sciences > 0604 Genetics > 060408 Genomics
06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070303 Crop and Pasture Biochemistry and Physiology
Socio-Economic Objective: B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820507 Wheat
Identification Number or DOI: 10.1111/j.1439-0434.2012.01956.x

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