Penton, C. Ryan and Gupta, V. V. S. R. and Tiedje, James M. and Neate, Stephen M. and Ophel-Keller, Kathy and Gillings, Michael and Harvey, Paul and Pham, Amanda and Roget, David K. (2014) Fungal community structure in disease suppressive soils assessed by 28S LSU gene sequencing. PLoS One, 9 (4). pp. 1-12.
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Abstract
Natural biological suppression of soil-borne diseases is a function of the activity and composition of soil microbial
communities. Soil microbe and phytopathogen interactions can occur prior to crop sowing and/or in the rhizosphere,
subsequently influencing both plant growth and productivity. Research on suppressive microbial communities has concentrated on bacteria although fungi can also influence soil-borne disease. Fungi were analyzed in co-located soils 'suppressive' or 'non-suppressive' for disease caused by Rhizoctonia solani AG 8 at two sites in South Australia using 454 pyrosequencing targeting the fungal 28S LSU rRNA gene. DNA was extracted from a minimum of 125 g of soil per replicate to reduce the micro-scale community variability, and from soil samples taken at sowing and from the rhizosphere at 7 weeks to cover the peak Rhizoctonia infection period. A total of 994,000 reads were classified into 917 genera covering 54% of the RDP Fungal Classifier database, a high diversity for an alkaline, low organic matter soil. Statistical analyses and
community ordinations revealed significant differences in fungal community composition between suppressive and nonsuppressive soil and between soil type/location. The majority of differences associated with suppressive soils were attributed to less than 40 genera including a number of endophytic species with plant pathogen suppression potentials and mycoparasites such as Xylaria spp. Non-suppressive soils were dominated by Alternaria, Gibberella and Penicillum. Pyrosequencing generated a detailed description of fungal community structure and identified candidate taxa that may influence pathogen-plant interactions in stable disease suppression.
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| Item Type: | Article (Commonwealth Reporting Category C) |
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| Refereed: | Yes |
| Item Status: | Live Archive |
| Additional Information: | Copyright: 2014 Penton et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Faculty/School / Institute/Centre: | Historic - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences (1 Jul 2013 - 5 Sep 2019) |
| Faculty/School / Institute/Centre: | Historic - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences (1 Jul 2013 - 5 Sep 2019) |
| Date Deposited: | 12 Jun 2014 04:25 |
| Last Modified: | 26 Aug 2015 02:54 |
| Uncontrolled Keywords: | DNA extraction; endophyte; fungal community; fungal plant disease; fungus identification; Fusarium; gene sequence; geographic distribution; LSU gene; microbial diversity; mycoparasite; Penicillium; plant fungus interaction; pyrosequencing; Rhizoctonia solani; rhizosphere fungus; RNA gene; soil analysis; Xylariales |
| Fields of Research (2008): | 06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology 05 Environmental Sciences > 0503 Soil Sciences > 050303 Soil Biology 06 Biological Sciences > 0605 Microbiology > 060504 Microbial Ecology |
| Socio-Economic Objectives (2008): | D Environment > 96 Environment > 9604 Control of Pests, Diseases and Exotic Species > 960413 Control of Plant Pests, Diseases and Exotic Species in Farmland, Arable Cropland and Permanent Cropland Environments |
| Identification Number or DOI: | https://doi.org/10.1371/journal.pone.0093893 |
| URI: | http://eprints.usq.edu.au/id/eprint/25187 |
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