Iterative germination and innovative techniques for the production and inoculation of secondary conidia of sorghum ergot (Claviceps africana)

Tonapi, V. A. and Ryley, M. and Galea, V. and Bhuiyan, S. and Wearing, A. (2009) Iterative germination and innovative techniques for the production and inoculation of secondary conidia of sorghum ergot (Claviceps africana). Archives of Phytopathology and Plant Protection, 42 (3). pp. 248-255. ISSN 0323-5408

Abstract

Five methods of harvesting pure secondary conidia were attempted, but only one was successful. The only successful method involved moving air across the surface of water agar plates or moist soil on which secondary sporulation was occurring at 0.2 – 3 m/s. Among the inoculation techniques, brushing the secondary conidia on to the wet stigma gave maximum ergot infection (80%) followed by brushing the
secondary conidia on to the dry stigma (77%). Conidia of Claviceps africana produced up to seven generations of sibling conidia. The length and width of secondary conidia showed a reduction in size from 10.92 – 10.13 mm and 5.49 – 5.13 mm, respectively, across generations. However no definite trend in decline in size of secondary conidia was evident. The optimum temperature range for germination of
secondary conidia of Australian isolates of C. africana is between 14.6 and 208C.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2009 Taylor & Francis. Published version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Historic - Faculty of Sciences - Department of Biological and Physical Sciences
Date Deposited: 05 Jun 2014 02:37
Last Modified: 02 Jul 2014 05:53
Uncontrolled Keywords: iterative; germination; ergot; sorghum (Sorghum bicolor (L.) Moench); secondary conidia; simple techniques
Fields of Research : 06 Biological Sciences > 0605 Microbiology > 060501 Bacteriology
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070305 Crop and Pasture Improvement (Selection and Breeding)
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Identification Number or DOI: 10.1080/03235400601036612
URI: http://eprints.usq.edu.au/id/eprint/25169

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