Spread of Fusarium pseudograminearum in wheat seedling tissues from a single inoculation point

Knight, N. L. and Sutherland, M. W. (2013) Spread of Fusarium pseudograminearum in wheat seedling tissues from a single inoculation point. Australasian Plant Pathology, 42 (5). pp. 609-615. ISSN 0815-3191

Abstract

Inoculation of wheat seedlings by the crown rot pathogen Fusarium pseudograminearum is commonly used to screen for resistance based on leaf sheath discolouration. While leaf sheath discolouration is effective for describing disease reactions, the degree to which the pathogen colonises the full range of seedling host tissues is an important aspect
of the disease process, which has not been systematically
examined. After single point droplet inoculation of the
coleoptile, quantitative PCR was used to determine the
spread of F. pseudograminearum into different host tissues
during crown rot pathogenesis at 14 and 28 days after
inoculation. Quantitative PCR and visual assessment indicated that while most seedling tissues (leaf sheath, leaf blade, sub-crown internode, primary root and secondary
root) were colonised, the basal portion of leaf sheath tissue supported the highest density of F.pseudograminearum.
Other significant sites of colonisation were the upper leaf
sheath and sub-crown internode. By comparison, the quantity
of mycelium detected in the primary and secondary roots was
significantly lower, while the infection of leaf blade tissues was severely restricted.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: Copyright Australasian Plant Pathology Society Inc. 2013. Published version deposited in accordance with the copyright policy of the publisher.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences
Date Deposited: 16 Sep 2013 02:11
Last Modified: 15 Jul 2014 01:49
Uncontrolled Keywords: crown rot; quantitative PCR
Fields of Research : 06 Biological Sciences > 0604 Genetics > 060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics)
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070302 Agronomy
07 Agricultural and Veterinary Sciences > 0703 Crop and Pasture Production > 070308 Crop and Pasture Protection (Pests, Diseases and Weeds)
Socio-Economic Objective: B Economic Development > 82 Plant Production and Plant Primary Products > 8205 Winter Grains and Oilseeds > 820507 Wheat
Identification Number or DOI: 10.1007/s13313-013-0225-z
URI: http://eprints.usq.edu.au/id/eprint/24048

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