The diversity and antimicrobial activity of Preussia sp. Endophytes isolated from Australian dry rainforests

Mapperson, Rachel R. and Kotiw, Michael and Davis, Rohan A. and Dearnaley, John D. W. (2014) The diversity and antimicrobial activity of Preussia sp. Endophytes isolated from Australian dry rainforests. Current Microbiology , 68 (1). pp. 30-37. ISSN 0343-8651

Abstract

Limited knowledge currently exists regarding species diversity and antimicrobial activity of endophytic isolates of Preussia within Australia. This report describes endophytic Preussia species that were identified through molecular analysis of the internal transcribed spacer region. Screening for antimicrobial secondary metabolites was determined by testing crude ethyl acetate (EtOAc) extracts derived from fungal mycelia against a panel of ATCC type strains which included Bacillus cereus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Serratia marcescens, methicillin-resistant Staphylococcus aureus (MRSA) and the opportunist yeast pathogen Candida albicans. Subsequently, high-performance liquid chromatography generated fractions of bioactive EtOAc extracts which were subject to confirmatory testing using the Clinical and Laboratory Standards Institute reference microdilution antimicrobial activity assay. A total of 18 Preussia were isolated from nine host plants with 6/18 having a <97% sequence similarity to other known species in Genbank, suggesting that they are new species. In preliminary screening, 13/18 Preussia isolates revealed antimicrobial activity against at least one of the microbes tested, whilst 6/18 isolates, including 4/6 putative new species showed specific antimicrobial activity against MRSA and C. albicans. These results highlight the antimicrobial potential of Australian Preussia spp. and also the importance of Australian dry rainforests as an untapped repository of potentially significant bioactive compounds.


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Item Type: Article (Commonwealth Reporting Category C)
Refereed: Yes
Item Status: Live Archive
Additional Information: © 2013 Springer Science+Business Media New York. Permanent restricted access to Published version due to publisher copyright policy.
Faculty / Department / School: Current - Faculty of Health, Engineering and Sciences - School of Agricultural, Computational and Environmental Sciences
Date Deposited: 03 Sep 2013 00:25
Last Modified: 12 May 2015 04:30
Uncontrolled Keywords: fungal mycelia; assay; rainforest plants; antimicrobial potential
Fields of Research : 06 Biological Sciences > 0607 Plant Biology > 060704 Plant Pathology
06 Biological Sciences > 0605 Microbiology > 060501 Bacteriology
03 Chemical Sciences > 0304 Medicinal and Biomolecular Chemistry > 030403 Characterisation of Biological Macromolecules
Socio-Economic Objective: E Expanding Knowledge > 97 Expanding Knowledge > 970106 Expanding Knowledge in the Biological Sciences
Identification Number or DOI: 10.1007/s00284-013-0415-5
URI: http://eprints.usq.edu.au/id/eprint/24006

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